In this visualized experiment, protocol details are provided for in vitro labeling of human embryonic stem cells (hESC) with second harmonic generation nanoparticles (HNPs). The latter are a new family of probes recently introduced for labeling biological samples for multi-photon imaging. HNPs are capable of doubling the frequency of excitation light by the nonlinear optical process of second h...

A new approach to alias-free wide-field fluorescence lifetime imaging in the frequency domain is demonstrated using a supercontinuum source for fluorescence excitation and a phase-modulated image intensifier for detection. This technique is referred to as phi-squared fluorescence lifetime imaging (phi(2)FLIM). The phase modulation and square-wave gating of the image intensifier eliminate aliasi...

We derive a two parameter multi-exponential model to describe the frequency spectrum of Barkhausen noise in bulk steel under high excitation rates and applied tensile stress. We show how the amplitude and shape of the frequency spectrum depend on two directly measurable quantities, Barkhausen voltage and effective magnetic permeability, respectively, and how these change with stress. By incorpo...

This article explores the excitation of different vibrational states in a spatially extended dynamical system through theory and experiment. As a prototypical example, we consider a one-dimensional packing of spherical particles (a so-called granular chain) that is subject to harmonic boundary excitation. The combination of the multi-modal nature of the system and the strong coupling between th...

The suppression of the regenerative chatter phenomenon in turning process via the variation of the frequency and amplitude of the spindle motor excitation is carried out in this research. A programmable inverter is employed for variable periodic excitation of the spindle motor. A set of turning experiments is designed and performed on a number of conical work pieces both in constant spindle spe...

Introduction: The recently described RASER sequence is a conceptual novelty in MR imaging [1]. Based on frequency-swept excitation, and timeencoding, RASER is a rapid, pure T2-weighted scheme without signal voids, ghosting and distortion artifacts present in conventional echo-planar imaging (EPI). These properties render it attractive for functional imaging (fMRI). However, in the previous impl...

a new multi-stage fuzzy (msf) controller based on multi-objective harmony search algorithm (mohsa) is proposed in this paper to solve the load frequency control (lfc) problem of power systems in deregulated environment. lfc problem are caused by load perturbations, which continuously disturb the normal operation of power system. the objectives of lfc are to mini small size the transient deviati...

We propose a method to control electromagnetic (EM) radiations by holographic metasurfaces, including to producing multi-beam scanning in one dimension (1D) and two dimensions (2D) with the change of frequency. The metasurfaces are composed of subwavelength metallic patches on grounded dielectric substrate. We present a combined theory of holography and leaky wave to realize the multi-beam radi...

Random lasers (RLs) based on neodymium ions (Nd(3+)) doped crystalline powders rely on multiple light scattering to sustain laser oscillation. Although Stokes and anti-Stokes Nd(3+) RLs have been demonstrated, the optical gain obtained up to now was possibly not large enough to produce self-frequency conversion. Here we demonstrate self-frequency upconversion from Nd(3+) doped YAl3(BO3)4 monocr...