نتایج جستجو برای: ms medium
تعداد نتایج: 357524 فیلتر نتایج به سال:
Optimizing regeneration of cotton plant in two variety (Sahel and Varamin) via shoot apex was done in order to Agrobacterium-mediated transformation. In this reaserch shoot apexes of two varieties were isolated from four or five day seedling and were placed on a special medium of shooting (modified MS without hormon).In order to select the best rooting media, The Statistical Analysis explants t...
sugar type and growth regulators are essential fundamental components of tissue culture media. in this study, different concentrations of some carbohydrate and growth regulators during anther culture and producing somatic embryo of datura stramonium l. was investigated in ms medium. the ms medium supplemented with 0.5 mgl-1 kinetin and 2 mgl-1 2,4-d, and also the medium supplemented with 0.25 m...
Petunia (Petunia hybrida) is an ornamental plant species of high economic potential. One of the appropriate methods for propagation and breeding of petunia is tissue culture. This experiment was conducted to investigate the effects of different concentrations of benzyl adenine (BA) either alone or in combination with different concentrations of naphthalene acetic acid (NAA) on the regeneration ...
In vitro regeneration of four cultivars of carnation namely ‘Nelson’, ‘Impulse’, ‘Sagres’ and ‘Spitit’ through somatic embryogenesis was studied. MS culture medium was supplemented with 30 gl-1 sucrose, 2 mgl-1 2,4-D and 0.2 mgl-1 BA and used for embryogenic callus formation. Somatic embryos were formed when embryogenic callus was transferred to MS medium without growth regulators containing 30...
gavane-gaz-angubin (persian manna) belongs to astragalus genus (leguminosae). this plant is one of the most important species in iran for its medicinal value. considering the low propagation rate of this plant from seed and cutting, the feasibility of in-vitro propagation of this plant was investigated. effect of different types and concentrations of cytokinins such as bap, kin, and ze (0.5, 1,...
this study was conducted with the main prupose of investigation the effects of culture medium and the concentration of growth regulators on proliferation rate, rooting and the acclimatization of gisela 6 rootstock at khorasan razavi agriculture and natural resource research center during 2011-2012. in this experiment, the proliferation and rooting were performed in six kinds of culture medium (...
punicagranatum cv. malassaveh is one of the most desirable commercial cultivars in iran. in thisstudy some experiments were conducted to introduce the best hormonal treatments for directadventitious shoot regeneration from mentioned cultivar via cotyledonary explants. for thispurpose, both liquid and agarmurashige and skoog (1962) (ms) medium supplemented with baat 2, 4.5, 9, 13 μm alone and in...
An efficient plant regeneration protocol was developed from leaf and internode (stem) explants of Bacopa monniera L. on MS basal medium, supplemented with Cytokinins like BAP, KN, and TDZ used singly. The leaf cultures expressed maximum plant regeneration potential with 138.9 ± 0.50 shoots per explants on MS + 2.0 mg/ l BAP. The in vitro regenerated dwarf shoots were further elongated on MS med...
The objective of this research was in-vitro germination and callus induction of Onosma bulbotrichum (O. bulbotrichum) as a medicinal herb which belongs to Boraginaceae family. For germination, the seedswere cultured on growth regulator-free MS medium and for callus induction, seeds were sown on modified MS medium containing different concentrations of kinetin (kn)- Indole-3-acetic acid (IAA) an...
in vitro propagation of az x m9 new apple rootstock resulted of breeding program of vegetative apple rootstock in iran was investigated. nodal explants were inoculated in murashige and skoog (ms) medium supplemented with various concentrations of 6-benzylaminon purine (bap) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (naa) (0.1 mg/l). we examined the effect of different concen...
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