Renal metabolism by UDP-glucuronosyltransferase (UGT) enzymes is central to the clearance of many drugs. However, significant discrepancies about the relative abundance and activity of individual UGT enzymes in the normal kidney prevail among reports, whereas glucuronidation in tumoral kidney has not been examined. In this study, we performed an extensive profiling of glucuronidation metabolism...

Cell-specific information on the quantity and localization of key mRNAs at single copy sensitivity in single cells is critical for evaluating basic cellular process, disease risk, and efficacy of therapy. Quantification of overexpressed mRNAs beyond the diffraction limit is constrained by the optical property of the probes and microscopy techniques. In this report, nanosized barium titanium oxi...

The study of cytokines gene expression is quite important in various conditions of health and disease for the evaluation of clinical responses to new vaccination approaches. An absolute quantification is based on a calibration curve and production of standard controls to achieve more reliable results than in relative system. In this study we attempted to construct standard controls to evaluate ...

the study of cytokines gene expression is quite important in various conditions of health and disease for the evaluation of clinical responses to new vaccination approaches. an absolute quantification is based on a calibration curve and production of standard controls to achieve more reliable results than in relative system. in this study we attempted to construct standard controls to evaluate ...

The article describes a polymerase chain reaction (PCR) based method for semiquantitative assessment of mRNA expression for chemotactic cytokines in the lower respiratory tract cells. Cytokine mRNA was detected using PCR preceded by reverse transcription of mRNA into cDNA. The procedures of RNA extraction and of reverse transcription-PCR assay are described in the first part of the article. The...