Various recent studies in both human and laboratory animals have indicated an influential role of glucose and phosphate in embryonic development of different strains and species in various media. So this study was initiated to investigate the effects of different concentrations of glucose (G0=0, Gl=0.2, G2=5.5 mM) in the absence (P0) and presence (P1=0.39 mM) of inorganic phosphate, designate...

Objective Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mor...

Cover illustration: The cover image is based on the Research Article Repetitive short-pulsed illumination efficiently activates photoactivatable-Cre as continuous in embryonic stem cells and pre-implantation embryos of transgenic mouse by Kanae Kishi et al., https://doi.org/10.1002/dvg.23457.

background: over the past several years, mammals have been successfully cloned by either the splitting of an early stage embryo or nuclear transfer of adult somatic cells (nt) into oocytes. although it has been 15 years since the generation of the first cloned mammals from somatic cells by nt, the success rate for producing live offspring by this technique is low regardless of the cell type and...

The changes in epigenetic modifications during early embryonic development significantly impact mammalian genome activation (EGA) and are species-conserved to some degree. Here, we reanalyzed the published RNA-Seq of human, mouse, goat embryos found that Zfp296 (zinc finger protein 296) expression was higher at EGA stage than oocyte all three species (adjusted p-value < 0.05 |log2(foldchange...

in order to do in vitro fertilization (ivf), 435 oocytes were collected from 20 super ovulated nmri female mice, in m2 media with 4% bsa. 85% (n=370) of collected oocytes were in mii phase. epididymal sperms were added to 332 oocytes in t6 media drops with 4% bsa and incubated. fertilization rate was 79.8% (n=265) out of which 27.5% (n=73) grew to morula and 20.8% (n=55) achieved blastocyst sta...

Domestic cat embryos of excellent quality appear to improve development of conspecific embryos when cultured together, providing an avenue for improving development of embryos from valuable species or individuals. To have relevance to rare species, it would be useful to understand if this advantage could be conferred by heterospecific companions because there usually are severely limited number...

Background: While assisted reproductive techniques (ARTs) are considered to be safe, many concerns exist about the safety of these techniques. Two important and indispensable parts of these techniques are in vitro culture and vitrification. While much progress has been made in developing culture conditions, it remains suboptimal. In order to investigate the effect of culture medium on in vitro ...