Three murine monoclonal anti-ssDNA autoantibodies have been produced by fusion of spleen cells from NZB/NZW F1 hybrid mice with Sp2/0-Ag 14 murine myeloma cells. Specificity studies characterizing monoclonal anti - ssDNA autoantibodies revealed binding patterns for nucleotides. Clone E-4-1 showed preferential binding with mono-di-and polyguanosine. Clones E-4-4 and E-11-1 specitically bound mon...

The concentrations of riboflavin and of the two coenzymes derived from riboflavin, flavin mononucleotide and flavin adenine dinucleotide (FAD), were determined in Novikoff hepatoma grown i.p. and in host liver of normal and riboflavin-deficient male Holtzman rats. Riboflavin deficiency significantly prolonged the 50% survival time of tumor-bearing animals from 5.7 to 10.8 days. The growth of No...

In the presence of flavin mononucleotide and light, methionine is nonenzymatically oxidized to carbon dioxide, formic acid, ethylene, methyl disulfide, and ammonia. Tracer studies indicate that these products arise, respectively, from carbon atoms 1, 2, 3, and 4, methyhnercapto, and amino groups of methionine. It is suggested that the f&t photochemical reaction is the oxidation of methionine to...

BACKGROUND Mononucleotide tracts in the coding regions of the TGFBR2 and BAX genes are commonly mutated in microsatellite instability-high (MSI-high) colon cancers. The receptor TGFBR2 plays an important role in the TGFB1 (transforming growth factor-β, TGF-β) signaling pathway, and BAX plays a key role in apoptosis. However, a role of TGFBR2 or BAX mononucleotide mutation in colorectal cancer a...

L-Amino acid oxidase has been crystallized from extracts of rat kidney mitochondria. Purification involved sonic extraction, ammonium sulfate fractionation, chromatography on diethylaminoethyl cellulose, and gel filtration through Sephadex G-ZOO. The enzyme revealed a single protein band on starch gel electrophoresis and exhibited a single homogeneous peak (a%, W = 10.5 S) in an ultracentrifuge...

The synthesis and isolation of two derivatives of 2-amino-1,3,4-thialdiazole(aminothiadiazole) are described. The derivatives are a nicotinamide adenine dinucleotide (NAD) analog prepared by an exchange reaction with NAD in the presence of nicotineamide adenine dinucleotide glycohydrolase and a presumed aminothiadiazole mononucleotide prepared by treatment of the NAD analog with nucleotide pyro...

Electron spin resonance measurements were performed for the reactions of some endogenous photosensitizers (flavin mononucleotide or flavin adenine dinucleotide or folic acid or β-nicotinamide adenine dinucleotide or β-nicotinamide adenine dinucleotide phosphate or pyridoxal-5'-phosphate or urocanic acid) with oleic acid under the ultraviolet light A irradiation using α-(4-pyridyl-1-oxide)-N-ter...

Experiments have shown that DNA can be sequenced using an electrolytic cell with a nanopore and an exonuclease enzyme in the cis chamber that cleaves the leading mononucleotide in a strand of DNA. The base therein can be identified with an accuracy of 80-90% by the level of the current blockade caused in the pore; a biological adapter inside slows down the cleaved mononucleotide and lowers the ...

Highly polymorphic and transferable microsatellites (SSRs) are important for comparative genomics, genome analysis and phylogenetic studies. Development of novel species-specific microsatellite markers remains a costly and labor-intensive project. Therefore, interest has been shifted from genomic to genic markers owing to their high inter-species transferability as they are developed from conse...