Monoclonal antibodies (mAbs) have well established their valuable roles as therapeutic proteins. Second generation of mAbs as therapeutical products for immunotherapy of HIV infection has made significant progress in recent studies. A recent small phase 1 clinical trial has demonstrated that monoclonal antibodies could be safe and effective in reducing HIV-1 viraemia in human. This study also s...

there are many studies related to the production of a elisa kit for diagnosing virus infections. however, production of most kits depends on purification of whole virus particles, which involves the use of costly equipment and reagents. the purpose of this study was to check out if the anti-cp42 antibodies could be used as a diagnostic assay for detection of grapevine fanleaf virus (gflv). in t...

the b-cell cd20 antigen is one of the most reliable surface targets in immunotherapy of b lymphoma. in this project, we studied the production and characterization of a new monoclonal antibody against chimeric human cd20 extra loops (hcd20 exl). the results showed that clone c12h, igg2/k isotype reacted with the antigen in elisa and immunoblot. the kd value was  found to be 2×10-9m and flow cyt...

Lack of response to monoclonal antibodies (mAbs) has been associated with inadequate mAb serum concentrations. Therapeutic drug monitoring (TDM) of mAbs has the potential to guide to more effective dosing in individual patients. This review discusses the mechanisms responsible for interpatient variability of mAb pharmacokinetics, summarizes exposure-response data of mAbs used in inflammatory an...

Batch release testing for human and veterinary tetanus vaccines still relies heavily on methods that involve animals, particularly potency testing. The quantity quality of antigen present in these products is utmost importance product safety clinical effect. Immunochemical measure consistency content quality, potentially as an indicator potency, could be a better choice negate the need vivo tes...

Due to the importance of monoclonal antibodies (mAb) as therapeutic agents, there is a growing demand for high resolution analytical methods to characterize these complex molecules. One important property of an mAb is its charge state that may change during and after manufacturing. A very useful technique to measure mAb charge heterogeneity is capillary isoelectric focusing (cIEF). This Applica...

Hydrophilic interaction chromatography (HILIC) coupled to mass spectrometry (MS) is considered as the reference analytical technique for glycans profiling, especially characterization of glycosylated protein therapeutics such monoclonal antibodies (mAbs) and mAbs-related products. Although HILIC/MS mainly known profile enzymatically released fluorescently labeled N-glycans, recent commercializa...

Broadly cross-reactive monoclonal antibodies define epitopes for vaccine development against HIV and other highly mutable viruses. Crystal structures are available for several such antibody-epitope complexes, but methods are needed to translate that structural information into immunogens that re-elicit similar antibodies. We describe a general computational method to design epitope-scaffolds in...

the production of murine monoclonal antibody (mab) has not yet been reported in iran. the present work describes for the first time the generation of several murine hybridoma clones secreting mabs directed against human leukocyte surface antigens. the secreted antibodies by hybridoma clones have been screened on different lymphoid and non-lymphoid tissues. results indicated that of seven hybrid...

Pancreatic cancer remains as one of the most aggressive types. In absence reliable biomarkers for its early detection and more effective therapeutic interventions, pancreatic is projected to become second leading cause death in Western world next decade. Therefore, it essential discover novel targets develop cancer-specific agents. To date, 45 monoclonal antibodies (mAbs) have been approved tre...