Interleukin-1 (IL-1) plays an important role in the pathophysiology of osteoarthritis (OA), and gene transfer of IL-1 receptor antagonist (IL-1Ra) holds promise for OA treatment. A preclinical safety and biodistribution study evaluated a self-complementary adeno-associated viral vector carrying rat IL-1Ra transgene (sc-rAAV2.5rIL-1Ra) at 5 × 10(8), 5 × 10(9), or 5 × 10(10) vg/knee, or human IL-...

BACKGROUND It was recently reported that the mono-iodoacetate (MIA) experimental model of osteoarthritis (OA) courses with changes of neurons innervating the affected joints that are commonly interpreted as a neuronal response to axonal injury. To better characterize these changes, we evaluated the expression of two markers of neuronal damage, ATF-3 and NPY, and the growth associated protein GA...

Citrate reversal of iodoacetate inhibition of glutamate synthesis is nonmetabolic. Reversal of fluoride inhibition is metabolic, occurring only at low Mg concentrations.

Osteoarthritis (OA) is a common, degenerative joint disease characterized by articular cartilage degradation. Currently, clinical trials based on microRNA therapy have been performed to treat various diseases. However, no treatment has been found for arthritis. This study investigated the functions of miR-101 in cartilage degradation in vivo and evaluated the feasibility of using miR-101 as a t...

1. A new method is described which gives a continuous record of the absolute rate of protoplasmic streaming in epidermal cells of the Avena coleoptile. 2. With this method a study was made of the influence of malate and iodoacetate on streaming velocity, in order to make correlations with the previously established effects of these substances on growth and respiration. 3. In the presence of opt...

In the study of reactions designed to identify the amino acid residues most directly involved in the catalytic function of pancreatic deoxyribonuclease, the enzyme was tist treated with iodoacetate under conditions similar to those which are effective in alkylating essential histidine residues in pancreatic ribonuclease; no inactivation of DNase occurred although some reagent was incorporated. ...

Carboxymethylation by iodoacetate of dihydrofolate reductase from the amethopterin-resistant mutant Streptococcus faecium var. Durans strain A leads to a loss of enzymic activity. Amino acid analysis showed that methionine is the only amino acid residue significantly affected by iodoacetate under the experimental conditions, and this was confirmed by the use of [ll’C]iodoacetate and ion exchang...

Carboxymethylation by iodoacetate of dihydrofolate reductase from the amethopterin-resistant mutant Streptococcus faecium var. Durans strain A leads to a loss of enzymic activity. Amino acid analysis showed that methionine is the only amino acid residue significantly affected by iodoacetate under the experimental conditions, and this was confirmed by the use of [1-14-C]iodoacetate and ion excha...

background : this study was set to determine the optimal technique of breast cancer with supraclavicular fossa irradiation. methods : three techniques was compared in this study. techniques were two point symmetric (2p-sym), two point asymmetric (2p-asym) and single point (sp). in 2p-sym all of beams were symmetric, in 2p-asym tangential beams were symmetric and supra was asymmetric, in sp all ...

ONE ofthe grounds on which disturbance of glycolysis has been assumed to be responsible for experimentally induced degeneration of the retina is the observation recorded by Noell (1951) that injection of sodium iodoacetate abolishes the electric response of the retina much more markedly in mammals than in other species. This finding is suggestive in view of the fact that the mammalian retina is...