ATP-driven translocation of helicases along DNA can be assayed in several ways. Reagentless biosensors, based on fluorophore-protein adducts, provide convenient ways for real-time assays of both the separation of dsDNA and the hydrolysis of ATP. Single-stranded DNA can be assayed using a modified single-stranded DNA-binding protein (SSB), and phosphate production during ATP hydrolysis can be me...

A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. The Lowry method ( Chapter 1 in vol. 1 of this series) has been widely used, but is susceptible to interference from a wide range of compounds commonly present in biological extracts. Although interference can be avoided by trichloracetic acid precipitation of the protein prior ...

ly referred to as L.S. Lowry, is remembered for being enigmatic and mischievous. Lowry, who rejected 5 different honors during his lifetime—including an Officer of the Most Excellent Order of the British Empire in 1955 and knighthood in 1968—holds the record for the most rejected British honors. Born in 1887, Lowry lived an upscale area of south Manchester, UK, until financial difficulties forc...

Schatz, G.E., P.P. Lowry II, H.N. Rakouth & R. Randrianaivo (2021). Taxonomic studies of Diospyros (Ebenaceae) from the Malagasy region. VI. New species large trees Madagascar. Candollea 76: 201–236. In English, English and French abstracts. An ongoing revision Ebenaceae region has focused in particular on potentially vulnerable to illegal exploitation for their hardwood. Eighty two c. 255 L. M...