The monapinone coupling enzyme (MCE), a fungal multicopper oxidase, catalyzes the regioselective C–C between tricyclic A (the primary substrate) and other monapinones (secondary substrates) to produce atropisomeric biaryl homo- or heterodimers. In this study, mono-, bi- compounds were tested determine whether they worked as secondary substrates for MCE. Among 14 cyclic compounds, MCE utilized s...

The economical profitability of the dairy industry is based on the quality of the bulk milk collected in the farms, therefore it was based on the herd level rather than on the individual animals at real time. Udder infection and stage of lactation are directly related to the quality of milk produced on the herd level. However, improvement of milk quality requires testing each animal's milk sepa...

Abstract Genetically engineered chymosin from the tree shrew ( Tupaia belangeri chinensis ) has been obtained and partially characterized for first time. The target enzyme was produced in Escherichia coli , strain BL21(DE3). It shown that recombinant coagulates cow milk Bos taurus ). total specific milk-clotting activity of 0.7–5.3 IMCU/mL 8.8–16.6 IMCU/mg. nonspecific proteolytic relation to b...

A gene for calf prochymosin (prorennin) has been reconstructed from chemically synthesized oligodeoxyribonucleotides and cloned DNA copies of preprochymosin mRNA. This gene has been inserted into a bacterial expression plasmid containing the Escherichia coli tryptophan promoter and a bacterial ribosome binding site. Induction of transcription from the tryptophan promoter results in prochymosin ...

Of 48 fungal isolates, 28 (including one Penicillium and three Aspergilli) secreted milk-clotting enzymes. This activity was in general related to the mycelial growth of a given isolate, but not with pH change of the culture filtrate during incubation. It was not found when mucilage was formed by isolates capable of doing so. The ratio of general proteolytic activity to milk-clotting activity v...