Aloe plants can be grown autotrophically in a maintenance-free, sterile, ex vitro environment. Excel, 15-5-15 plus minors fertilizer formulation and Plant Preservative Medium (PPM) biocide was used as a very economical basal medium. In vitro micropropagated plantlets were regenerated in Excel media with 2 g /L sucrose and 2.2 μM 6-Benzylamino purine (BA) and used to initiate ex vitro cultures i...

Individual lines of Vitis vinifera Barbera and Chardonnay were obtained by in vitro culture of axillary buds collected from phytoplasma-affected mother plants. The molecular assays did not detect the “flavescence dorée” (FD) phytoplasma in the 66 lines tested after 6-9 months of culture, while the “bois noir”-associated phytoplasma was detected in 22 out of 50 lines. Despite the observed ineffi...

AIMS To evaluate the colonization process of sugarcane plantlets and hydroponically grown rice seedlings by Gluconacetobacter diazotrophicus strain PAL5 marked with the gusA and gfp reporter genes. METHODS AND RESULTS Sugarcane plantlets inoculated in vitro with PAL5 carrying the gfp::gusA plasmid pHRGFPGUS did not present green fluorescence, but beta-glucuronidase (GUS)-stained bacteria coul...

To evaluate the effect of plastic film coverage on ex vitro acclimatization arrow cane (Gynerium sagitatum Aubl.), shoots “Criolla”, Martinera” and “Costera” cultivars were in micropropagated a medium supplied with 6-Benzylaminopurine half them rooted 1-Naphtalene acetic acid. Rooted unrooted transplanted shade house fog irrigation, into trays (72 clusters per tray), using peat as substrate was...

Pyrethrum is a perennial herbaceous plant endemic to the eastern coast of Adriatic Sea, and introduced in large areas nearly all continents, where it cultivated for industrial extraction pyrethrins. Pyrethrins are group six closely related monoterpene esters, widely used as natural insecticides. The world production pyrethrins lower than market demand, wider introduction this crop within Medite...