BACKGROUND A multicentre study was carried out in Ghana and southern India to determine the aetiology of suppurative keratitis in two regions located at similar tropical latitudes. Studies of fungal keratitis from the literature were reviewed. METHODS Patients presenting at rural and urban eye units with suspected microbial keratitis were recruited to the study. Corneal ulceration was defined...

Barriers that rapidly oppose potential corneal pathogens include innate immunity, tears that form a barrier and contain antimicrobial substances, and blink reflex that helps to spread tears over the cornea and mechanically sweep away pathogens. These protective mechanisms are controlled by nerves that can both stimulate and inhibit immune functions, stimulate tear production, and regulate blink...

OBJECTIVES Microbial keratitis accounts for up to 30% of blindness in some less developed societies. The development of a single broad-spectrum topical antimicrobial effective against bacteria, fungi and Acanthamoeba would have a major impact on reducing the morbidity and simplifying the treatment of microbial keratitis. To this end, the activity of the amidoamine myristamidopropyl dimethylamin...

Background: Microbial keratitis is a potentially sight threatening disorder and the leading cause of monocular blindness worldwide. This study was conducted to find out the causes of microbial keratitis in our set up. Patients & Methods: It was a prospective study performed at Augenklinik, Khyber Medical Centre, Peshawar, from January 2007 to December 2009. Patients with infective corneal ulcer...

PURPOSE This study aimed to determine the effect of salicylic acid on the membrane proteome, sensitivity to antibiotics, and production of microbial keratitis by Pseudomonas aeruginosa. METHODS P. aeruginosa 6294 was grown in the presence or absence of 30 mM salicylic acid. Bacterial membrane proteins were extracted in carbonate buffer, separated using two-dimensional gel electrophoresis and ...

Identification of Acanthamoeba cysts and trophozoites in ocular tissues requires considerable expertise and is often time-consuming. An 18S rRNA gene-based PCR test, highly specific for the genus Acanthamoeba, has recently been reported in the molecular diagnosis of Acanthamoeba keratitis. This PCR assay was compared with conventional microbiological tests for the diagnosis of Acanthamoeba kera...