The use of mass spectrometry imaging for crystallinity detection offers improved matrix selectivity and sensitivity over the techniques, such as X-ray microscopy Raman spectrometry, that are traditionally used with this work. Crystallinity is observed in electrospray laser desorption ionization (ELDI-MSI) a high-intensity agglomeration analyte spatially resolved image. As an indirect method det...

The Gene Expression Viewer is a web-launched three-dimensional visualization tool, tailored to compare surface reconstructions of multi-channel image volumes generated by confocal microscopy or micro-CT.

The lamina cribrosa (LC) is a tissue in the posterior eye with a complex trabecular microstructure. This tissue is of great research interest, as it is likely the initial site of retinal ganglion cell axonal damage in glaucoma. Unfortunately, the LC is difficult to access experimentally, and thus imaging techniques in tandem with image processing have emerged as powerful tools to study the micr...

Volume averaging results in both over- and underestimation of airway dimensions when they are measured by high-resolution computed tomography (HRCT). The current authors calibrated computerised measurements of airway dimensions from HRCT against a novel three-dimensional micro-computed tomography (CT) standard, which has a 50-fold greater resolution, as well as against traditional morphometry. ...

Background: Cone-beam computed tomography (CBCT) scanners for image-guided radiotherapy are in clinical use today, but there has been no consensus on uniform acceptance to verify the CBCT image quality yet. The present work proposed new methods to fully evaluate the performance of CBCT in its three-dimensional (3D) reconstruction space. Materials and Methods: Compared to the traditional methods...

It is commonly postulated that local mechanics (tissue stress or strain) is related to stimuli for bone remodeling [1]. A micro finite element ( FE) solver was developed in our lab to numerically predict the local stress and strain in bone structure [2,3]. Micro-computed tomography ( CT) can provide high resolution image data suitable to the FE solver and allows non-destructive, three-dimension...

Understanding of the failure composites is critical for evaluating their safety and reliability when they are in service. In this research, a combined image analysis technique using computed microtomography (micro-CT) scanning electron microscopy (SEM) proposed to study microstructures, damage hybrid unidirectional/woven composite laminate (HUWCL). Micro-CT used extract 3D microscale morphology...

Objective: To optimize scan time and X-ray dose with no loss of image quality for retrospectively-gated micro-CT scans of free-breathing rats. Methods: Five free-breathing rats were scanned using a dynamic micro-CT scanner over 10 continuous gantry rotations (50 seconds and entrance dose of 0.28 Gy). The in-phase projection views were selected and reconstructed, representing peak inspiration an...