The particular bacterium under investigation here (S. pasteurii) is unique in its ability, under the right conditions, to induce the hydrolysis of urea (ureolysis) in naturally occurring environments through secretion of an enzyme urease. This process of ureolysis, through a chain of chemical reactions, leads to the formation of calcium carbonate precipitates. This is known as Microbiologically...

This paper aims at monitoring the improvement of sandy soil properties with biocementation through microbially induced calcite precipitation (MICP) method reaction accelerations by self-developed soybean urease enzymes. In this study, concentration calcium ions (Ca2+ as CaCl2) is varied 50, 100, 250, and 500 mM to determine an optimum shear strength. The enzymes 20% volume (v/v) are used accele...

AIMS Ureolysis drives microbially induced calcium carbonate precipitation (MICP). MICP models typically employ simplified urea hydrolysis kinetics that do not account for cell density, pH effect or product inhibition. Here, ureolysis rate studies with whole cells of Sporosarcina pasteurii aimed to determine the relationship between ureolysis rate and concentrations of (i) urea, (ii) cells, (iii...

Peat is one of the most challenging and problematic soils in fields geotechnical environmental engineering. The critical problems related to peat are extremely low strength high compressibility, resulting poor inhabitancy infrastructural developments their vicinity. Thus far, were stabilized using Portland cement; however, production cement causes significant emission greenhouse gases, which no...

Microbial Induced Carbonate Precipitation (MICP) has recently become a new technology for stabilizing the slope surface. The applicability of MICP, however, is limited in fine-grained soils due to restrictions regarding transportation bacteria cells. purpose this study was assess feasibility an alternative called Bacterial-Enzyme (B-EICP) soils. Unlike MICP strategy (involving whole-cells bacte...

Microbial-induced carbonate precipitation (MICP) has been explored for more than a decade as promising soil improvement technique. However, it is still challenging to predict and control the growth rate characteristics of CaCO3 precipitates, which directly affect engineering performance MICP-treated soils. In this study, we employ microfluidics-based pore-scale model observe effect bacterial de...