The pharmacokinetics of the methotrexate enterohepatic cycle were studied in rats in vivo. For plasma levels of methotrexate between 10(-5) and 10(-8) M, biliary levels were directly proportional and concentrated 27-fold. When labeled methotrexate was administered in doses sufficient to achieve plasma levels of 10(-6) M, approximately 50% of methotrexate appeared in the bile in normal animals a...

The folate analog, methotrexate, binds very tightly to dihydrofolate reductase. However, free intracellular methotrexate in excess of the enzyme-binding capacity is needed to suppress cellular dihydrofolate reduction, and the effects of methotrexate are rapidly reversible if methotrexate is removed from the extracellular media. The apparent paradox may be explained on the basis of a single high...

OBJECTIVE To determine whether two adults with stress fractures receiving low weekly doses of methotrexate had methotrexate osteopathy. CASE REPORTS Two adult patients developed features consistent with methotrexate osteopathy while receiving low weekly doses of methotrexate. METHODS Iliac crest biopsy samples were taken and bone histomorphometry carried out. RESULTS Symptoms resolved whe...

Hypersensitivity reactions to methotrexate are rare, but have been reported. Methotrexate has shown activity against many malignancies, and omission of methotrexate therapy may increase the risk of cancer-related death in some patients. Therefore, rechallenging patients with methotrexate following hypersensitivity may be beneficial. We report a case of a child with metastatic osteosarcoma who e...

Aim: Methotrexate, a folic acid antagonist, is chemotherapeutic drug used in the treatment of various inflammatory diseases as well some cancer types.The purpose this study; It study effects boric against hepatotoxic side methotrexate.
 Materials and Methods: Male wistar albino rats were divided into five groups six animals each. The group 1 control group. Methotrexate was administered to ...

Methotrexate is the most commonly used drug in the treatment of rheumatoid arthritis, although 30-40% of patients fail to adequately respond. An accurate method for measuring methotrexate polyglutamates, the stable active metabolite of methotrexate, has recently been described. The objective of this review article is to determine if clinical use of this measurement would improve methotrexate ef...

The basis for the proliferation-dependent cytotoxicity of methotrexate has been investigated in mice bearing the L5178Y ascites leukemia. Methotrexate at 60 mg/kg i.p. reduced the viability of logarithmically growing ascites cells (55% active S phase cells) to 28% of control, whereas the viability of the slowly growing cells (18% active S phase) was decreased to only 59% of control. Log phase t...

The basis for the proliferation-dependent cytotoxicity of methotrexate has been investigated in mice bearing the L5178Y ascites leukemia. Methotrexate at 60 mg/kg i.p. reduced the viability of logarithmically growing ascites cells (55% active S phase cells) to 28% of control, whereas the viability of the slowly growing cells (18% active S phase) was decreased to only 59% of control. Log phase t...

abortion is an important problem in obstetrics throughout the world. the common and standard method for pregnancy termination at first trimester is surgery (curettage). nowadays, an effective method of pregnancy termination at first trimester is medical treatments. the aim of this study is to compare misoprostol alone or in combination with methotrexate for pregnancy termination at first trimes...

OBJECTIVE To compare the efficacy and safety of treatment with infliximab plus methotrexate with methotrexate alone in methotrexate-naive patients with active psoriatic arthritis (PsA). METHODS In this open-label study, patients 18 years and older with active PsA who were naive to methotrexate and not receiving disease-modifying therapy (N=115) were randomly assigned (1:1) to receive either i...