Pseudomonas putida S-313 can utilize a broad range of aromatic sulfonates as sulfur sources for growth in sulfate-free minimal medium. The sulfonates are cleaved monooxygenolytically to yield the corresponding phenols. miniTn5 mutants of strain S-313 which were no longer able to desulfurize arylsulfonates were isolated and were found to carry transposon insertions in the ssuEADCBF operon, which...

Background: The search for potent radioprotective agents for the amelioration of radiation side effect is an important aim in radiobiology. The present study aimed to evaluate the effects of curcumin and seleno-L-methionine against radiation-induced micronucleus formation in rat bone marrow.Methods: In total, 40 male rats were divided into 8 groups (n=5 each), including control, curcumin or sel...

The objectives of this study were to evaluate ruminal degradation and intestinal digestion of two ruminally protected methionine (RPM) products and to assess the potential use of changes in plasma methionine concentrations as an indication of methionine availability to the animal. Ruminal degradation of the protected methionine was assessed using the in situ technique. The intestinal availabili...

in order to determination the methionine requirement of arian broilers chickens during the starter period, a trial with 700 broiler chickens in a completely randomized design with 7 treatments, 5 replicates and 20 chicks in each experimental units was performed. experimental groups were consisted of 0.38, 0.43, 0.48, 0.53, 0.58, 0.63 and 0.68  % of methionine. other amino acids provided higher ...

Holstein steers were used in two 5 x 5 Latin square experiments to evaluate the sparing of methionine by alternative sources of methyl groups (betaine and choline). Steers were housed in metabolism crates and limit-fed a soybean hull-based diet high in rumen degradable protein. To increase energy supply, ruminal infusions of volatile fatty acids and abomasal infusions of glucose were provided. ...

The mechanism of formation of the methyl group of methionine is of particular interest at the present time since the reaction may be studied in cell-free systems and is dependent on the direct participation of vitamin Bit. The reported systems for study of methionine biosynthesis in vitro have been derived from mammalian, avian, and bacterial sources (&lo). Serine and formaldehyde have been the...

The mechanism of formation of the methyl group of methionine is of particular interest at the present time since the reaction may be studied in cell-free systems and is dependent on the direct participation of vitamin Bit. The reported systems for study of methionine biosynthesis in vitro have been derived from mammalian, avian, and bacterial sources (&lo). Serine and formaldehyde have been the...