Coastal regions are potential zones for production of methane which could be governed by ecological/environmental differences or even sediment properties of a niche. In order to test the hypothesis that methanogenesis in most marine sediments could be driven more by proteins than by carbohydrates and lipid content of labile organic matter (LOM), incubation experiments were carried out with sedi...

Four in vitro experiments (Exp.) were conducted to evaluate lavender essential oil (LEO) effects at 0 (control), 250 (low dose), 500 (medium dose), 750 and 1000 µL per L (high doses) of incubation medium on rumen gas production kinetics (Exp.1), ruminal digestibility and fermentation (Exp.2), methane production (Exp.3) and rumen acidosis (Exp.4). The asymptote of gas production (A) increased qu...

The effect of grape pomace (Vitis vinifera) and in oak leaf (Quercus libani) tannins by three runs of in vitro gas production on the organic matter digestibility (OMD) and in situ dry matter (DM) and crude protein (CP) disappearance were studied in four sheeps. Polyethylene glycol (PEG) was used to deactivate the tannins. In vitro gas production was recorded at 2, 4, 6, 8, 12, 48, 72, 96 and 12...

Two trials were conducted to identify the optimal levels of essential oil active components (EOAC) and their combination with fumarate on in vitro rumen fermentation. In trial 1, eugenol, carvacrol, citral and cinnamaldehyde were mixed at ratios of 1:2:3:4, 2:1:4:3, 3:4:1:2, 4:3:2:1 and 1:1:1:1 to make up five combinations (EOAC1, EOAC2, EOAC3, EOAC4 and EOAC5 respectively). The mixtures were s...

This study was conducted to compare the methane (CH4) production estimated by in vivo (sulfur hexafluoride tracer technique (SF6)) with that of two in vitro rumen simulation (RUSITEC) and gas production (IVGPT)) techniques. Four adult dry Holstein cows, aged 7.4±3.0 years and weighing 697±70 kg, were used for measuring methane production from five diets by the SF6 technique. The experimental di...

theaim of the current study was to assess effects of different levels of origanum vulgare (0, 250, 500, 750 and 1000 mg/l) on kinetics of gas production, some ruminal digestion and fermentation parameters, methane production and also to determine its potential in controlling rumen acidosis using in vitro method in four separate phases. in the first phase, the asymptote of gas production (a) and...

Aim: The present study was done to evaluate the effect of Myristica fragrans fruit active compounds addition on methane production in vitro. Materials and Methods: Methanolic extract of Myristica fragrans fruit powder was prepared and checked for its inhibitory action on methane production in diet containing roughage 50 percent and concentrate 50 percent respectively. Methane production was est...

We have developed a technique for cultivation of chemolithoautotrophs under high hydrostatic pressures that is successfully applicable to various types of deep-sea chemolithoautotrophs, including methanogens. It is based on a glass-syringe-sealing liquid medium and gas mixture used in conjunction with a butyl rubber piston and a metallic needle stuck into butyl rubber. By using this technique, ...