The present investigation was carried out to study the In vitro shoot multiplication and callogenesis in Gladiolus. For this purpose, cormel, meristem and leaf explants were chosen. Different concentrations of BAP i. e., 0.5, 1.0, 1.5, 2.0 or 2.5 mgL (2.22, 4.44, 6.66, 8.88 or 11.10 μM) were supplemented to MS medium. Both cormel and meristem explants showed maximum multiplication on MS medium ...

Stress-adaptive cell plasticity in target tissues and cells for plant biomass growth is important for yield stability. In vitro systems with reproducible cell plasticity can help to identify relevant metabolic and molecular events during early cell reprogramming. In carrot, regulation of the central root meristem is a critical target for yield-determining secondary growth. Calorespirometry, a t...

The strategy for mass cloning of plants by micro­ propagation depends on disease-indexing the donor plant, and eliminating any diseases found, before setting up the explant or meristem cultures. Details of tissue culture media have been published for most commercially important species [1]. Fungal, bacterial and mycoplasmal pathogens can be successfully elim­ inated by the addition of the appro...

This study was aimed to develop a simple, comprehensive and efficiently repetitive protocol for micropropagation of banana (Musa sapientum L.) using shoot meristem. After growing in-vitro cultures on different hormonal combinations, Murashige and Skoog’s medium supplemented with BAP and IAA (5.0 + 1.0 mg/l, respectively) and 10% CW were found to be most efficient and productive combination for ...

The control of organ growth by coordinating cell proliferation and differentiation is a fundamental developmental process. In plants, postembryonic root growth is sustained by the root meristem. For maintenance of root meristem size, the rate of cell differentiation must equal the rate of cell division. Cytokinin and auxin interact to affect the cell proliferation and differentiation balance an...

a methodology to regenerate whole plants of fig tree (ficus carica cv. jaami-e-kan) from apical meristem of greenhouse plantlets was developed. two meristematic explants size (0.2-0.4 and 0.5-0.7 mm), two kinds of culture media and three concentrations of 6-benzyladenine (0.5, 1 and 1.5 mgrl-1) were tested as a completely randomize design (crd) with a simple factorial arrangement for establishm...

To characterize the SHL2 and SHL1 genes in detail, we analyzed three strains carrying weak alleles of SHL2, shl2-6, shl2-7, and shl2-8, and one weak allele of SHL1, shl1-3. In contrast to strong alleles, which result in lack of shoot meristem, strains bearing these weak alleles formed shoot meristem frequently during embryogenesis. In shl2-6 and shl2-7 mutants, the meristem was lost during seed...

The shoot apical meristem contains a pool of undifferentiated stem cells and controls initiation of all aerial plant organs. In maize (Zea mays), leaves are formed throughout vegetative development; on transition to floral development, the shoot meristem forms the tassel. Due to the regulated balance between stem cell maintenance and organogenesis, the structure and morphology of the shoot meri...

Shoot and floral meristem activity in higher plants is controlled by complex signaling networks consisting of positive and negative regulators. The Arabidopsis ULTRAPETALA1 (ULT1) gene has been shown to act as a negative regulator of meristem cell accumulation in inflorescence and floral meristems, as loss-of-function ult1 mutations cause inflorescence meristem enlargement, the production of ex...

The control of organ growth by coordinating cell proliferation and differentiation is a fundamental developmental process. In plants, postembryonic root growth is sustained by the root meristem. For maintenance of root meristem size, the rate of cell differentiation must equal the rate of cell division. Cytokinin and auxin interact to affect the cell proliferation and differentiation balance an...