Objectives: This study was designed to investigate the potential Phyllanthus niruri extracts in promotion of hair growth. Materials and Methods: Here, we studied the hair growth promoting activity of petroleum ether extract of P. niruri following its topical administration. Alopecia was induced in albino rats by subcutaneous administration of testosterone for 21 days. Evaluation of hair loss in...

The reaction between the FAD-containing enzyme, mercuric reductase, and its reducing substrate, NADPH, has been studied at 5 degrees C, pH 7.3, by rapid-scan and fixed-wavelength stopped-flow techniques with the aim to characterize reaction intermediates. With an excess of NADPH the spectral changes observed in rapid-scan experiments can be described by a simple kinetic model, A----B----C----D,...

The effects of substrates and inhibitors on the conformation of yeast cytoplasmic pyruvate decarboxylase (Z-oxo-acid carboxy-lyase, EC 4 .l .l .l) has been examined using the technique of automated tritium-hydrogen exchange. This experimental approach, as used in this study, reflects primarily the number of solvent-inaccessible or “core” hydrogens in the protein. Yeast cytoplasmic pyruvate deca...

The tellurium oxyanion tellurite (TeO3 (2-)) is extremely harmful for most organisms. It has been suggested that a potential bacterial tellurite resistance mechanism would consist of an enzymatic, NAD(P)H-dependent, reduction to the less toxic form elemental tellurium (Te(0)). To date, a number of enzymes such as catalase, type II NADH dehydrogenase and terminal oxidases from the electron trans...

Penicillinase plasmids of Staphylococcus aureus often contain genes conferring resistance to inorganic mercury (Hg(2+)) and the organomercurial phenylmercury acetate. The mechanism of resistance was found to be the enzymatic hydrolysis of the organomercurial phenylmercury to benzene plus inorganic ionic mercury, which was then enzymatically reduced to metallic mercury (Hg(0)). The Hg(0) was rap...

objectives: this study was designed to investigate the potential phyllanthus niruri extracts in promotion of hair growth. materials and methods: here, we studied the hair growth promoting activity of petroleum ether extract of p. niruri following its topical administration. alopecia was induced in albino rats by subcutaneous administration of testosterone for 21 days. evaluation of hair loss in...

Within ten days of incubation, freshly ripe tomato fruits (Ibadan local variety) obtained from a local market in Ile-Ife, Osun State, Nigeria had completely deteriorated and proteins which possessed cellulolytic activity were released. The enzyme was partially purified by a combination of ammonium sulphate precipitation, molecular exclusion chromatography and ion-exchange chromatography. The en...

Mercuric reductase, MerA, is a key enzyme in bacterial mercury resistance. This homodimeric enzyme captures and reduces toxic Hg2+ to Hg0, which is relatively unreactive and can exit the cell passively. Prior to reduction, the Hg2+ is transferred from a pair of cysteines (C558' and C559' using Tn501 numbering) at the C-terminus of one monomer to another pair of cysteines (C136 and C141) in the ...