نتایج جستجو برای: menten equation

تعداد نتایج: 232097  

2015
Michael J. Lawson Linda Petzold Andreas Hellander

Quantitative biology relies on the construction of accurate mathematical models, yet the e↵ectiveness of these models is often predicated on making simplifying approximations that allow for direct comparisons with available experimental data. The Michaelis-Menten approximation is widely used in both deterministic and discrete stochastic models of intracellular reaction networks, due to the ubiq...

Journal: :Biochemistry 2011
Leonor Michaelis Maud Leonora Menten Kenneth A Johnson Roger S Goody

Nearly 100 years ago Michaelis and Menten published their now classic paper [Michaelis, L., and Menten, M. L. (1913) Die Kinetik der Invertinwirkung. Biochem. Z. 49, 333-369] in which they showed that the rate of an enzyme-catalyzed reaction is proportional to the concentration of the enzyme-substrate complex predicted by the Michaelis-Menten equation. Because the original text was written in G...

Journal: :Drug metabolism and disposition: the biological fate of chemicals 1999
T Kakkar H Boxenbaum M Mayersohn

There are a variety of methods available to calculate the inhibition constant (Ki) that characterizes substrate inhibition by a competitive inhibitor. Linearized versions of the Michaelis-Menten equation (e.g., Lineweaver-Burk, Dixon, etc.) are frequently used, but they often produce substantial errors in parameter estimation. This study was conducted to compare three methods of analysis for th...

Journal: :Biophysical chemistry 2016
Wylie Stroberg Santiago Schnell

The conditions under which the Michaelis-Menten equation accurately captures the steady-state kinetics of a simple enzyme-catalyzed reaction is contrasted with the conditions under which the same equation can be used to estimate parameters, KM and V, from progress curve data. Validity of the underlying assumptions leading to the Michaelis-Menten equation are shown to be necessary, but not suffi...

Journal: :Nature 1961

Journal: :Accounts of chemical research 2005
Wei Min Brian P English Guobin Luo Binny J Cherayil S C Kou X Sunney Xie

Recent single-molecule enzymology measurements with improved statistics have demonstrated that a single enzyme molecule exhibits large temporal fluctuations of the turnover rate constant at a broad range of time scales (from 1 ms to 100 s). The rate constant fluctuations, termed as dynamic disorder, are associated with fluctuations of the protein conformations observed on the same time scales. ...

Journal: :Biopharmaceutics & drug disposition 2015
Hirotaka Miyamoto Satoshi Matsueda Akihiro Moritsuka Kenta Shimokawa Haruna Hirata Mikiro Nakashima Hitoshi Sasaki Shintaro Fumoto Koyo Nishida

The effect of hypothermia on the in vivo pharmacokinetics of midazolam was evaluated, with a focus on altered metabolism in the liver and binding to serum proteins. Rat primary hepatocytes were incubated with midazolam (which is metabolized mainly by CYP3A2) at 37, 32 or 28 °C. The Michaelis-Menten constant (Km) and maximum velocity (Vmax) of midazolam were estimated using the Michaelis-Menten ...

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