methods mycobacterium tuberculosis standard strain crbip7.11 was cultured at 37°c in loewenstein johnson (lj) media for 3-4 weeks. to confirm the species, standard microbiological and biochemical tests were performed. after preparation of membrane vesicles, the amount of protein in membrane vesicles was measured by sds-page and nanodrop. to analyze the integrity and morphology of extracellular ...

The aim of this investigation was to assess the effect of different antioxidants on the motility andmembrane integrity of the ram semen during storage at 5ºC for 0, 12, 24, 48 and 72 hrs after collection anddilution. A total of 57 ejaculates from 12 Bakhtiary rams was collected by artificial vagina and were extended1 : 3 with egg yolk-citrate buffer, which was containing one of the following an...

experiment was conducted to evaluate the effect of different levels of egg yolk for coating and storing spermatozoa. ejaculates were collected from four rams. in each session, second ejaculates (n=4) were collected in a tube containing 1 ml coating buffers which were prepared by 10, 15 or 20% egg yolk plus tris-fructose. samples were pooled, centrifuged and the supernatant removed in the labora...

Semen quality and its relationship to fertility are major concern in animal production. The aim of this study was to assess the relationship between frozen thawed sperm characteristics and fertility following in vivo fertility through Artificial Insemination(AI). Semen samples were collected from four buffalo bulls. Semen volume and sperm concentration appeared to be significantly different (P0...

The effects of Ag(I) and Hg(II) on membrane potential and integrity of cells of Candida albicans and C. maltosa were determined with a flow cytometric procedure that employed an anionic membrane potential-sensitive dye, bis-(1,3-dibutylbarbituric acid) trimethine oxonol, and a membrane integrity indicator, propidium iodide. The membrane potentials of cells of both species were reduced rapidly w...

the aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (bubalus bubalis) bulls. five healthy buffalo bulls (5 ejaculates from each bull) were used. each ejaculate was diluted at 37 ˚c with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 μg ml-1 sodium selenite and the sperm motility and...