This laboratory study examined viability and infectivity of the entomopathogenic nematode (EPN) Heterorhabditis bacteriophora Poinar in solutions containing four different turfgrass soil surfactants: Revolution (Aquatrols Corp., Paulsboro, NJ), Aqueduct (Aquatrols Corp., Paulsboro, NJ), Cascade Plus (Precision Laboratories Inc., Waukegan, IL) and OARS (Aqua-Aid Inc., Rocky Mount, NC). Infective...

Daily irrigated, 80% pan replacement, and nonirrigated field plots of Kentucky bluegrass (Poa pratensis L.) were inoculated with a mixture of Steinernema carpocapsae (All strain) and Heterorhabditis bacteriophora (HP-88 strain) in 1988. In 1989, daily irrigated and nonirrigated plots were inoculated with HP-88 alone. The turf and associated soil contained populations of Tylenchorhynchus dubius,...

The effect of Steinernema riobrave and Heterorhabditis bacteriophora on population density of Mesocriconema xenoplax in peach was studied in the greenhouse. Twenty-one days after adding 112 M. xenoplax adults and juveniles/1,500 cm(3) soil to the soil surface of each pot, 50 infective juveniles/cm(2) soil surface of either S. riobrave or H. bacteriophora were applied. Another entomopathogenic n...

The optimum temperature for embryogenesis in Meloidogyne javanica lies between 25 and 30 C. Embryogenesis is slightly more rapid at 30 C (9-10 days), but more eggs complete development at 25 C (11-13 days). At temperatures of 25, 27.5, and 30 C, embryogenesis is about twice as rapid as at 20 C (23-25 days), and about four times as rapid as at 15 C (46-48 days). Time-lapse studies showed that th...

The potential impacts on natural enemies of crops that produce insecticidal Cry proteins from Bacillus thuringiensis (Bt) are an important part of an environmental risk assessment. Entomopathogenic nematodes are important natural enemies of lepidopteran pests, and the effects of Bt crops on these nontarget organisms should be investigated to avoid disruption of their biological control function...

Entomopathogenic nematodes (EPNs) in the genera Steinernema and Heterorhabditis and their associated bacteria (Xenorhabdus spp. and Photorhabdus spp., respectively) are lethal parasites of soil dwelling insects. We collected 168 soil samples from five provinces, all located in southern Thailand. Eight strains of EPNs were isolated and identified to species using restriction profiles and sequenc...

Heterorhabditis bacteriophora Project Summary: This project aims to sequence the genome of the entomopathogenic nematode Heterorhabditis bacteriophora strain TTO1 as a representative of 45 other species that are used as biological control agents for insect pests worldwide. This nematode is closely related to the two already sequenced free-living nematodes, Caenorhabditis elegans and C. briggsae...

Effects of culture filtrate of Verticillium chlamydosporium were evaluated on mortality of larvae and hatching of eggs of Meloidogyne javanica. Mortality of M. javanica larvae was found to be directly proportional to concentrations of culture filtrate of V. chlamydosporium and duration of exposure. Maximum mortality of larvae (65.5%) was observed in 100% concentration of culture filtrate after ...