نتایج جستجو برای: meca protein

تعداد نتایج: 1236161  

2011
Laura García-Álvarez Matthew TG Holden Heather Lindsay Cerian R Webb Derek FJ Brown Martin D Curran Enid Walpole Karen Brooks Derek J Pickard Christopher Teale Julian Parkhill Stephen D Bentley Giles F Edwards E Kirsty Girvan Angela M Kearns Bruno Pichon Robert LR Hill Anders Rhod Larsen Robert L Skov Sharon J Peacock Duncan J Maskell Mark A Holmes

BACKGROUND Animals can act as a reservoir and source for the emergence of novel meticillin-resistant Staphylococcus aureus (MRSA) clones in human beings. Here, we report the discovery of a strain of S aureus (LGA251) isolated from bulk milk that was phenotypically resistant to meticillin but tested negative for the mecA gene and a preliminary investigation of the extent to which such strains ar...

Journal: :The Southeast Asian journal of tropical medicine and public health 2002
Taweeporn Siripornmongcolchai Chariya Chomvarin Kunyaluk Chaicumpar Temduang Limpaiboon Chaisiri Wongkhum

Detection of the mecA gene by polymerase chain reaction (PCR) is the gold standard for identifying methicillin-resistant Staphylococcus aureus (MRSA). PCR assays, employing MR1-MR2 primers (primer set 1) and MR3-MR4 primers (primer set 2) to generate 154 and 533 bp fragment, respectively, are most widely used for amplification of mecA gene. The purpose of this study was to evaluate the presence...

Journal: :Physiological research 2012
M Hofer M Pospíšil L Dušek Z Hoferová D Komůrková

In our previous studies, IB-MECA, an adenosine A(3) receptor agonist, was found to stimulate proliferation of hematopoietic progenitor and precursor cells in mice. This property of IB-MECA was considered to be responsible for its ability to support regeneration of suppressed hematopoiesis after irradiation with sublethal doses of γ-rays when the drug was given in a post-irradiation treatment re...

Journal: :Antimicrobial agents and chemotherapy 2008
Michael J Noto Paige M Fox Gordon L Archer

Treatment of infections caused by Staphylococcus aureus is often confounded by the bacterium's ability to develop resistance to chemotherapeutic agents. Methicillin-resistant S. aureus (MRSA) arises through the acquisition of staphylococcal chromosomal cassette mec (SCCmec), a genomic island containing the methicillin resistance determinant, mecA. In contrast, resistance to vancomycin can resul...

Journal: :Journal of clinical microbiology 1999
W B van Leeuwen C van Pelt A Luijendijk H A Verbrugh W H Goessens

The slide agglutination test MRSA-Screen (Denka Seiken Co., Niigata, Japan) was compared with the mecA PCR ("gold standard") for the detection of methicillin resistance in Staphylococcus aureus. The MRSA-Screen test detected the penicillin-binding protein 2a (PBP2a) antigen in 87 of 90 genetically diverse methicillin-resistant S. aureus (MRSA) stock culture strains, leading to a sensitivity of ...

Journal: :Indian journal of pathology & microbiology 2015
Oyebode Armstrong Terry Alli David O Ogbolu Adebayo O Shittu Anthony N Okorie John O Akinola John B Daniel

INTRODUCTION Staphylococcus aureus is the etiological agent for a wide range of human infections, and its pathogenicity largely depends on various virulence factors associated with adherence, evasion of the immune system and damage of the host. This study determined the prevalence of methicillin-resistant S. aureus (MRSA) and some selected virulence genes in clinical isolates of S. aureus from ...

Journal: :Journal of clinical microbiology 1998
C P Kolbert J Arruda P Varga-Delmore X Zheng M Lewis J Kolberg D H Persing

The identification of methicillin-resistant staphylococcus isolates in the clinical laboratory has typically been performed by using methods that detect phenotypic expression of resistance determinants. However, these methods may be difficult to interpret and some isolates do not express resistance until selective pressure is administered. Assays that detect genetic determinants are not subject...

2014
Ewan M. Harrison Gavin K. Paterson Matthew T. G. Holden Xiaoliang Ba Joana Rolo Fiona J. E. Morgan Bruno Pichon Angela Kearns Ruth N. Zadoks Sharon J. Peacock Julian Parkhill Mark A. Holmes

OBJECTIVES Methicillin resistance in Staphylococcus spp. results from the expression of an alternative penicillin-binding protein 2a (encoded by mecA) with a low affinity for β-lactam antibiotics. Recently, a novel variant of mecA known as mecC (formerly mecALGA251) was identified in Staphylococcus aureus isolates from both humans and animals. In this study, we identified two Staphylococcus sci...

2015
Hossein Motamedi Seyyed Soheil Rahmat Abadi Seyyed Mojtaba Moosavian Maryam Torabi

BACKGROUND Staphylococcus aureus, an important human pathogen is one of the main causative agents of nosocomial infection. Virulence genes play a major role in the pathogenicity of this agent and its infections. Methicillin-Resistant Staphylococcus aureus (MRSA) isolates are major challenge among infectious agents that can cause severe infections and mortality. Methicillin-resistant S. aureus p...

Journal: :The European respiratory journal 2009
A Nakou M Woodhead A Torres

S taphylococcus aureus is a species of bacterium that can cause a broad variety of infections, ranging from minor skin infections to severe pneumonia and sepsis. The genetic adaptation of S. aureus has led to a multidrug-resistant pathogen, meticillin-resistant S. aureus (MRSA) after the introduction of meticillin (previously methicillin) into clinical practice in the 1960s [1]. MRSA is resista...

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