OBJECTIVES This study focused on the correlation between geno- and phenotypic tests in the correct assessment of mecA-mediated methicillin resistance among coagulase-negative staphylococci (CoNS) and the further characterization of mecA-positive isolates. METHODS A total of 121 CoNS from cases of bovine mastitis were investigated for oxacillin susceptibility by disc diffusion and broth microd...

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) can cause serious and life-threatening hospital- and community-acquired infections. Colonized and infected patients represent the most important reservoir of MRSA in health care facilities. Therefore, in this study, MRSA isolates collected from Shohada Hospital in Tabriz were evaluated for the frequency of mecA gene and their anti...

In this study, Staphylococcus aureus was found to be the causative agent of furunculosis in 64 (27.5%) out 233 Iraqi patients presented with furunculosis. 16SrRNA gene located all isolates. Nevertheless, mecA and lukS-lukF genes were 60% 4% S. isolates, respectively. Interestingly, carrying isolates positive as well.

Recently, we have demonstrated that the cognate regulatory locus of the mecA gene in methicillin-resistant Staphylococcus aureus (MRSA) is in fact a three-component system containing the novel mecR2 gene coding for an antirepressor. MecR2 interacts with the repressor MecI, disturbing its binding to the mecA promoter and fostering its proteolysis. Here, we engineered a point mutation in the puta...

Staphylococcus aureus is colonized in the human nasal cavity and would contaminate hospital and therapeutic environments. This bacterium has a genetic diversity in terms of resistance to antimicrobial agents. Therefore, the purpose of this study was identificatied of Methicillin and Vancomycin resistant genes in Staphylococcus aureus strains which has been isolated from the nasal cavity. 189 pa...

Methicillin-resistant Staphylococcus aureus (MRSA) is an important human pathogen, which is cross-resistant to virtually all β-lactam antibiotics. MRSA strains are defined by the presence of mecA gene. The transcription of mecA can be regulated by a sensor-inducer (MecR1) and a repressor (MecI), involving a unique series of proteolytic steps. The induction of mecA by MecR1 has been described as...

We previously characterized over 100 Staphylococcus sciuri isolates, mainly of animal origin, and found that they all carried a genetic element (S. sciuri mecA) closely related to the mecA gene of methicillin-resistant Staphylococcus aureus (MRSA) strains. We also found a few isolates that carried a second copy of the gene, identical to MRSA mecA. In this work, we analyzed a collection of 28 S....

Treatment of infections caused by Staphylococcus aureus is often confounded by the bacterium's ability to develop resistance to chemotherapeutic agents. Methicillin-resistant S. aureus (MRSA) arises through the acquisition of staphylococcal chromosomal cassette mec (SCCmec), a genomic island containing the methicillin resistance determinant, mecA. In contrast, resistance to vancomycin can resul...