نتایج جستجو برای: meca

تعداد نتایج: 1758  

Journal: :Journal of clinical microbiology 2016
M T Wu C-A D Burnham L F Westblade J Dien Bard S D Lawhon M A Wallace T Stanley E Burd J Hindler R M Humphries

Staphylococcus pseudintermedius is a coagulase-positive species that colonizes the nares and anal mucosa of healthy dogs and cats. Human infections with S. pseudintermedius range in severity from bite wounds and rhinosinusitis to endocarditis; historically, these infections were thought to be uncommon, but new laboratory methods suggest that their true incidence is underreported. Oxacillin and ...

Journal: :Clinical and experimental rheumatology 2006
L Rath-Wolfson S Bar-Yehuda L Madi A Ochaion S Cohen A Zabutti P Fishman

OBJECTIVES The anti-inflammatory effect of adenosine is partially mediated via the A3 adenosine receptor (A3AR), a Gi protein associated cell surface receptor. The highly selective A3AR agonist, IB-MECA was earlier shown to prevent the clinical and pathological manifestations of arthritis in experimental animal models of collagen and adjuvant induced arthritis (AIA). In this study we tested the...

Journal: :The Journal of Experimental Medicine 2003
Christine M'Rini Guiying Cheng Colleen Schweitzer Lois L. Cavanagh Roger T. Palframan Thorsten R. Mempel Richard A. Warnock John B. Lowe Elizabeth J. Quackenbush Ulrich H. von Andrian

Lymphocytes home to peripheral lymph nodes (PLNs) via high endothelial venules (HEVs) in the subcortex and incrementally larger collecting venules in the medulla. HEVs express ligands for L-selectin, which mediates lymphocyte rolling. L-selectin counterreceptors in HEVs are recognized by mAb MECA-79, a surrogate marker for molecularly heterogeneous glycans termed peripheral node addressin. By c...

Journal: :Journal of clinical microbiology 2000
I Couto I S Sanches R Sá-Leão H de Lencastre

We previously characterized over 100 Staphylococcus sciuri isolates, mainly of animal origin, and found that they all carried a genetic element (S. sciuri mecA) closely related to the mecA gene of methicillin-resistant Staphylococcus aureus (MRSA) strains. We also found a few isolates that carried a second copy of the gene, identical to MRSA mecA. In this work, we analyzed a collection of 28 S....

Journal: :Journal of clinical microbiology 2001
R Zbinden M Ritzler E Ritzler B Berger-Bächi

In a recent article, Hussain et al. evaluated a commercial latex agglutination test (LA), the MRSA-Screen (Denka Seiken Co., Niigata, Japan), for rapid detection of penicillinbinding protein 2a (PBP2a) in mecA-positive and mecA-negative coagulase-negative staphylococci (CNS) (2). The sensitivity and specificity were 100 and 99.5% (2), respectively, although the MRSA-Screen was initially develop...

Journal: :Polish journal of microbiology 2006
Tomasz Nowak Ewa Balcerczak Marek Mirowski Eligia M Szewczyk

The aim of this study was to evaluate methicillin resistance detection methods currently used when studying coagulase-negative staphylococci (CONS). The resistance to oxacillin of 142 strains from seven species of CoNS isolated from the Intensive Care Unit environments was tested. The methods used were: disc diffusion test with cefoxitin (FOX30) and oxacillin (OX1), oxacillin agar screen test w...

Journal: :The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases 2008
Leandro Reus Rodrigues Perez Pedro Alves d'Azevedo

In this study, we determined the accuracy of phenotypic tests (cefoxitin agar dilution, 30 microg-cefoxitin and 1 microg-oxacillin disks) to detect the oxacillin resistance/mecA gene among coagulase-negative staphylococci (CoNS) isolates. The presence of the mecA gene was detected by PCR technique (gold standard). A total of 176 CoNS isolates from blood of hospitalized patients were evaluated. ...

Journal: :Journal of clinical microbiology 1994
M Shimaoka M Yoh A Segawa Y Takarada K Yamamoto T Honda

A DNA hybridization method with an enzyme-labeled oligonucleotide probe (mecA-ELONP) was developed to detect the methicillin-resistant gene (mecA) in methicillin-resistant Staphylococcus aureus. For rapid identification, bacterial colonies were transferred from agar plates directly onto nylon membranes. Lysis of cells, denaturation of DNA, and hybridization were performed on the membranes. Thes...

Journal: :Skin pharmacology and physiology 2016
Rutger Matthes Anne Lührman Silva Holtfreter Julia Kolata Dörte Radke Nils-Olaf Hübner Ojan Assadian Axel Kramer

Previous studies on the antimicrobial activity of cold atmospheric pressure argon plasma showed varying effects against mecA+ or mecA-Staphylococcus aureus strains. This observation may have important clinical and epidemiological implications. Here, the antibacterial activity of argon plasma was investigated against 78 genetically different S. aureus strains, stratified by mecA, luk-P, agr1-4, ...

Journal: :Antimicrobial agents and chemotherapy 2015
Liana C Chan Li Basuino Binh Diep Stephanie Hamilton Som S Chatterjee Henry F Chambers

The role of mecA mutations in conferring resistance to ceftobiprole and ceftaroline, cephalosporins with anti-methicillin-resistant Staphylococcus aureus (MRSA) activity, was determined with MRSA strains COL and SF8300. The SF8300 ceftaroline-passaged mutant carried a single mecA mutation, E447K (E-to-K change at position 447), and expressed low-level resistance. This mutation in COL conferred ...

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