Cysteine-rich secretory protein 3 (CRISP-3; also known as SGP28) was originally discovered in human neutrophilic granulocytes. We have recently developed a sensitive sandwich enzyme-linked immunosorbent assay for CRISP-3 and demonstrated the presence of CRISP-3 in exocrine secretions. To investigate the subcellular localization and mobilization of CRISP-3 in human neutrophils, we performed subc...

Chromogranin A (CgA) is transported restrictedly to secretory granules in neuroendocrine cells. In addition to pHand Ca2 -dependent aggregation, CgA is known to bind to a number of vesicle matrix proteins. Because the binding-prone property of CgA with secretory proteins may be essential for its targeting to secretory granules, we screened its binding partner proteins using a yeast two-hybrid s...

Low-pH- and calcium-induced aggregation of regulated secretory proteins has been proposed to play a role in their retention and storage in secretory granules. However, this has not been tested for secretory proteins that are stored in the exocrine parotid secretory granules. Parotid granule matrix proteins were analyzed for aggregation in the presence or absence of calcium and in the pH range o...

The secretory acinar cells of parotid glands from rats of varying ages have been examined by electron microscopy to determine what age-related changes occur in these cells. The most prominent change noted in these cells is the progressive increase in the amount of lipofuscin granules with age. Lipofuscin granules are membrane-bound structures consisting of lipids, other subcomponents, and a mat...

Chromogranin A (CgA) is transported restrictedly to secretory granules in neuroendocrine cells. In addition to pH-and Ca 2 +-dependent aggregation, CgA is known to bind to a number of vesicle matrix proteins. Because the binding-prone property of CgA with secretory proteins may be essential for its targeting to secretory granules, we screened its binding partner proteins using a yeast two-hybri...

This paper focuses on how statistical independence can be observed in a contingency table when the table is viewed as a matrix. Statistical independence in a contingency table is represented as a special form of linear dependence, where all the rows or columns are described by one row or column, respectively.

Colloidal gold was used as a marker for immunoelectron microscopy to localize lactoferrin (LF) and myeloperoxidase (MPO) in human peripheral blood neutrophils. Cells were reacted with monospecific antibodies against LF or MPO and then with gold-labeled antiglobulin. MPO cytochemistry was also associated with immunologic detection of LF. Immunologic labeling of thin sections after embedding in g...

A new direction in the development of metal-matrix coatings are composites based on an amorphous matrix with reinforcing ceramic additives. In this work, composite were prepared by electrospark treatment AISI 1045 a mixture iron granules multicomponent composition CrMoWCBSi and addition tungsten carbide powder. According to X-ray analysis, increase concentration WC up 1.2 vol.%, content phase c...

The mandibular glands of the Chinese hamster were examined by light and electron microscopy. The mandibular acinar cells were noted to be categorized in a seromucous type, because the acinar cells stained slightly basophile and moderately positive for periodic acid-Schiff (PAS) and alcian blue (AB) reactions and the intercellular canaliculi were detected in the cells. The acini were composed ex...

a controlled release matrix formulation for mesalamine was designed and developed to achieve a 24 h release profile. using compritol 888 ato (glyceryl behenate) as an inert matrix-forming agent to control the release of mesalamine, formulation granules containing the solid dispersions were investigated. pectin, a polysaccharide, was used as bacterial dependent polymer for colon targeting. the m...