Agglutination of yeast, human group A and guinea pig erythrocytes by multiple clinical isolates of E. coli, K. pneumoniae, and P. aeruginosa was investigated and correlated with hydrophobicity measurements of each bacterial strain. Hydrophobicity of the isolates, as measured by hydrophobic interaction chromatography, was similarly correlated with in vitro adherence of the microorganisms to bucc...

We investigated the hemolytic activity of Escherichia coli strain EC901 carrying plasmid pBJN406 containing genes draA-E involved in expression of the mannose-resistant Dr hemagglutinin, and in its isogenic insertion mutants devised with Tn5, Tn3, and TnphoA. While E. coli BN406 displayed rapid hemolytic activity against equine erythrocytes, insertion mutations in draD and draE, but not in draA...

Broadly neutralizing antibodies have been isolated that bind the glycan shield of the HIV-1 envelope spike. One such antibody, PGT135, contacts the intrinsic mannose patch of gp120 at the Asn332, Asn392, and Asn386 glycosylation sites. Here, site-specific glycosylation analysis of recombinant gp120 revealed glycan microheterogeneity sufficient to explain the existence of a minor population of v...

Mannose-resistant hemagglutination (MRHA) was evaluated for identification of Escherichia coli with K99 fimbriae. The sensitivity and specificity of MRHA, relative to the enzyme-linked immunosorbent assay, were 21 and 79%, respectively. Disagreement between the tests may have been due in part to separation of pili from the cells, with resultant enzyme-linked immunosorbent assay-positive, MRHA-n...

The extracellular acid phosphatase (EC 3.1.3.2) of Rhodotomla glutinis is a glycoenzyme composed of 608 protein, 35% mannose, and 5% N-acetylglucosamine by weight. The native enzyme elutes from a column of BioGel A-5M agarose with an M, 5 185,000 but on sodium dodecyl sulfate-acrylamide gel electrophoresis migrates with an M, = 93,000. The acid phosphatase thus appears to consist of two subun...

Hemocyanins are used as immunomodulators in clinical applications because they induce a strong Th1-biased cell-mediated immunity, which has beneficial effects. They multiligand glycosylated molecules with abundant and complex mannose-rich structures. It remains unclear whether these structures influence hemocyanin-induced immunostimulatory processes human APCs. We have previously shown that hem...