A major protease present in dormant bark tissues of the apple (Malus domestica Borkh. cv. "Golden Delicious") was partially purified by hemoglobin-coupled Sepharose column chromatography. The protease active at pH 4.6 and at temperatures of 30 to 50 C was found to be sulfhydryl-dependent. Phenylmercuric acetate inhibited the enzyme approximately 50% at 2 millimolar, whereas phenylmethylsulfonyl...

Effects of 1-methylcyclopropene (1-MCP), 2-chloroethyl phosphonic acid (ethephon), and methyl jasmonate (MeJA) on production of aroma volatile compounds and ethylene by ‘Delicious’ and ‘Golden Delicious’ apples [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.] during ripening were investigated. Forty-four volatile compounds in ‘Delicious’ and 40 compounds in ‘Golden Delicious’ were d...