Functional group transformations at the group 4 metallocene framework have been demonstrated, which have provided relatively straightforward access to otherwise synthetically challenging derivatives. The pendant nitrile group in Ti and Zr metallocene complexes of the type [(η(5)-C5Me5)(η(5)-C5H4CMe2CH2CN)MCl2] was converted into an intramolecularly bound ketimido moiety by alkylation, which too...

Treatment of murine leukaemia virus reverse transcriptase with benzophenone 4-maleimide inactivates DNA polymerase activity, but has no effect on the RNAase H function. Kinetic measurements indicated that benzophenone 4-maleimide is a competitive inhibitor with respect to template-primer binding, but is non-competitive with respect to dNTP binding. Enzyme modified with benzophenone 4-maleimide ...

14 S W Y : The incorporation of [ C ] N-ethylmaleimide reveals fast and slowreacting sulfhydryl groups in sarcoplasmic reticulum. Two proteins react with the label: a fast-reacting glycoprotein recently isolate$+(Ikemoto, Cucchiaro and Garcia (1976) J. Cell Biol. 10, 290a), and the Ca -ATPase. Labeling sarcoplasmic reticulum with a maleimide spin label gives a similar pattern. The spectra of ma...

Introducing various pendant functional groups and building blocks of interest to polypeptides in a highly efficient, controlled manner is crucial to access polypeptide materials with desired structures and functions. In this study, we synthesized γ-(4-vinylbenzyl)-(L)-glutamate N-carboxyanhydride (VB-Glu-NCA), which was readily obtained and purified in large quantity. VB-Glu-NCA monomer was sub...

The chemical properties of showdomycin, a maleimide type antibiotic, were very similar to, but its biological properties rather different from, those of N-ethylmaleimide. Another maleimide derivative, citraconimide, showed very low reactivity both in chemical and biological reactions compared with the above two compounds. The results of quantitative analyses of cellular sulfhydryl content of Es...

BACKGROUND Success of spinal anesthesia in pregnant females is strongly influenced by the position of the patient at the time of spinal insertion. Pendant position is a new position introduced to increase the intervertebral spaces. The current study aimed at comparing successful spinal puncture among the patients with pendant position in comparison with the ones with traditional sitting positio...

Recently, several thermogelling materials have been developed for biomedical applications. In this study, we prepared methoxy polyethylene glycol (MPEG)-b-(poly(ε-caprolactone)-ran-poly(2chloride-ε-caprolactone) (PCL-ran-PfCL)) (MP-Cl) diblock copolymers at room temperature via the ring-opening polymerization of caprolactone (CL) and 2-chloride-ε-caprolactone (fCL) monomers, using the terminal ...

Cyclic peptide architectures can be easily synthesized from cysteine-containing peptides with appending maleimides, free or protected, through an intramolecular Michael-type reaction. After peptide assembly, the peptide can cyclize either during the trifluoroacetic acid treatment, if the maleimide is not protected, or upon deprotection of the maleimide. The combination of free and protected mal...

The direct synthesis of dithiophenol maleimide functional polymers by living radical polymerisation is described without the need for protecting group chemistry. The synthesised polymers have been successfully employed as disulfide bridging agents for salmon calcitonin when used in equimolar quantities, negating the requirement for complex purification strategies, traditionally associated with ...