Peptide mass fingerprinting (PMF) by matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) provides a simple and direct means to unequivocally confirm identity of recombinant proteins based on predicted peptide profiles. Many universities or research institutions now carry mass spectrometry instrumentation as part of their core bioanalytical facilities or p...

This year marks the 20th anniversary of Koichi Tanaka securing Nobel Prize in chemistry, shared with John Fenn and Kurt Wüthrich, for demonstrating applicability laser technology to analyze biological macromolecules. The principle desorption is fundamental many today’s analytical methods such as matrix-assisted desorption/ionization (MALDI). In this article, we provide an overview MALDI highlig...

Sequencing of N-terminally blocked proteins/peptides is a challenge since these molecules inhibit processing by Edman degradation. By using high accuracy Fourier transform ion cyclotron resonance (FTICR) tandem mass spectrometry and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS), the primary structures of two novel N-terminally blocked antifungal pept...