OBJECTIVE Envenomation by heamotoxic snakes constituted a critical health occurrence in the world. Bleeding is the most sever consequence following snake bite with viperid and crothalid snakes. It is believed that the degradation of vascular membrane caused hemorrhage; in contrast, some suggested that direct cytotoxicity has role in endothelial cell disturbances. This study was carried out to e...

Snake venoms contain four classes of metalloproteases that all have a typical zinc-chelating sequence (HEXXHGXXH). N-terminal sequences and internal sequences of different purified metalloproteases were determined using Edman sequencing and LC MS/MS technique. Oligonucleotides were designed and used as primers for cDNA cloning from Vipera lebetina venom gland cDNA library. We found that isoform...

Background: Poisoning due to the bites and stings of venomous snakes and scorpions is a neglected public health problem, particularly in rural areas. Poor health facilities and inadequate knowledge of health care personnel are the major factors that result in envenomated human victims not receiving adequate care and medical attention. There is a great need for up-to-date and effective healthcar...

In this study, the venom extract of five viper species (Vipera xanthina, Vipera ammodytes, Vipera kaznakovi, Vipera wagneri and Vipera lebetina) collected from various regions of Turkey was subjected to polyacrylamide disc electrophoresis. No pressure was applied to the venom glands to provide venom extract. Important differences were detected among the electrophoretic patterns of venom protein...

Objective: Envenomation by heamotoxic snakes constituted a critical health occurrence in the world. Bleeding is the most sever consequence following snake bite with viperid and crothalid snakes. It is believed that the degradation of vascular membrane caused hemorrhage; in contrast, some suggested that direct cytotoxicity has role in endothelial cell disturbances. This study was to evaluate the...

abstract background and objective: snake venom is a complex of several toxic elements and enzymes. it has the agents with the ability to destroy cellular and subcellular membrane and to bring about hemolysis of red blood cells (rbc). two types of direct and indirect hemolytic activity are known in snake venom in that phospholipase a2 is responsible for the indirect lysis. the aim of this study ...

background snake venom is a complex mixture of different components including proteins and peptides that mostly has enzymatic properties. in this study, the alkaline phosphatase (alp) activity is measured in iranian vipera lebetina, venomous snake on sephadex g-100 to investigate the suitability of this procedure. material and methods the method of this study is experimental. 100 mg of the crud...