Metabolism and excretion of erlotinib, an orally active inhibitor of epidermal growth factor receptor tyrosine kinase, were studied in healthy male volunteers after a single oral dose of [14C]erlotinib hydrochloride (100-mg free base equivalent, approximately 91 microCi/subject). The mass balance was achieved with approximately 91% of the administered dose recovered in urine and feces. The majo...

Malignant melanoma (MM) is a highly malignant skin tumor. The mechanism of MM pathogenesis and its signaling pathways are not well characterized. C-X-C chemokine receptor type 7 (CXCR7) has been reported to regulate cancer cell invasion. The present study sought to investigate the effects of CXCR7 on MM development. First, CXCR7 expression levels were assessed in the skin tumor tissue of patien...

Introgressive hybridization has played a crucial role in crop breeding. Powdery mildew-resistant gene 21 (Pm21), located on the short arm of chromosome 6V of Haynaldia villosa (H. villosa), is typically a representative element which is transferred to hexaploid wheat through introgressive hybridization. Determination of sequence evidence for H. villosa DNA introgression would help trace Pm21 in...

A murine model was used to determine whether neutralizing monoclonal antibodies (MAbs) with heterotypic specificity directed to VP7 (MAb 57-8) or to the VP8 fragment of VP4 (MAb M14) passively protect mice against challenge with various strains of rotavirus. (The gene 4 product, an outer capsid protein, has traditionally been called VP3. It has been proposed, however, that the rotavirus gene 4 ...

Here we investigate the mechanism(s) involved in the c-Myc-dependent drug response of melanoma cells. By using three M14-derived c-Myc low-expressing clones, we demonstrate that alkylating agents, cisplatin and melphalan, trigger apoptosis in the c-Myc antisense transfectants, but not in the parental line. On the contrary, topoisomerase inhibitors, adriamycin and camptothecin, induce apoptosis ...

Hematopoietic growth factor receptors are present on cells of normal nonhematopoietic tissues such as endothelium and placenta. We previously demonstrated functional human granulocyte-macrophage colony-stimulating factor (GM-CSF) receptors on small cell carcinoma of the lung cell lines, and others have reported that certain solid tumor cell lines respond to GM-CSF in clonogenic assays. In the c...

The influence lead metal on DNA integrity in leaf plant can measure by potential effects of pollutantsaccumulation metal, Pb Phragmites australis was subjected to RAPD analysis, have been foundmetal at concentrations S1, S2,S3 (0.045 , 2.97, 0.93) Respectively. polymorphisms became evident asthe presence and absence fragments affected samples compared effect samples. Five primewas selected OP-L...

Exponentially growing human melanoma cells (M14 cell line) were pretreated with various amounts of dipalmitoylphosphatidylcholine-containing multilamellar liposomes and then exposed to heat treatment (42.5 degrees C). Cell damage produced by the treatments, given separately or in combination, was evaluated in terms of cell survival. Our results demonstrate that the cell survival at 37 degrees C...

Image segmentation plays an important role in medical image processing. The aim of conventional hard segmentation methods is to assign a unique label to each voxel. However, due to the limited spatial resolution of medical imaging equipment and the complex anatomic structure of soft tissues, a single voxel in a medical image may be composed of several tissue types, which is called partial volum...