Abstract Hydrothermal methods are commonly applied in pretreatment of lignocellulose for conversion to biofuels and chemicals. This partially disassembles solubilizes hemicelluloses lignin, releasing sugars phenolic compounds, which potential inhibitors the subsequent enzymatic saccharification step. In this work, inhibitory effect soluble phenolics hydrothermal liquor on canonical Trichoderma ...

Lytic granule exocytosis is the major pathway used by CD8+ CTL to kill virally infected and tumor cells. Despite the obvious importance of this pathway in adaptive T cell immunity, the molecular identity of enzymes involved in the regulation of this process is poorly characterized. One signal known to be critical for the regulation of granule exocytosis-mediated cytotoxicity in CD8+ T cells is ...

Cultured bovine aortic endothelial cells (BAEC) released endothelin-1 (ET-1) in the culture medium in a time-dependent fashion. Coincubation of fMLP-activated human polymorphonuclear leukocytes (PMN) with BAEC caused a fast (maximal activity was reached within 15 minutes) and cell number-dependent disappearance of ET-1 from the medium. This effect was direct to ET-1, because it was also present...

The C-terminal domain of Escherichia coli YfhD functions as a lytic transglycosylase* Edie M. Scheurwater and Anthony J. Clarke From the Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1 Canada Running head: E. coli membrane-bound lytic transglycosylase F Address correspondence to: Anthony J. Clarke, Department of Molecular and Cellular Biology, Univers...

Molecular-genetic and muropeptide analysis techniques have been applied to examine the function in vivo of the Bacillus megaterium QM B1551 SleB and SleL proteins. In common with Bacillus subtilis and Bacillus anthracis, the presence of anhydromuropeptides in B. megaterium germination exudates, which is indicative of lytic transglycosylase activity, is associated with an intact sleB structural ...

13 Correspondent footnote: 14 Department of Biological Sciences 15 Virginia Tech 16 Life Sciences I MC0910 17 Washington St. 18 Blacksburg, VA 24061 19 Phone: (540) 231-2529. Fax: (540) 231-4043. E-mail: [email protected] 20 Copyright © 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. J. Bacteriol. doi:10.1128/JB.01380-09 JB Accepts, published on...

Mycoparasitic Trichoderma spp. act as potent biocontrol agents against a number of plant pathogenic fungi, whereupon the mycoparasitic attack includes host recognition followed by infection structure formation and secretion of lytic enzymes and antifungal metabolites leading to the host's death. Host-derived signals are suggested to be recognized by receptors located on the mycoparasite's cell ...

Our laboratory has developed phage lytic enzymes to prevent infection by specifically destroying disease bacteria on mucous membranes and in blood. Enzymes specific for S. pneumoniae and S. pyogenes have been developed to be used nasally and orally to control these organisms in environments such as hospitals and nursing homes to prevent or markedly reduce serious infections by these pathogens. ...