approved: ____________________________________________________________ Adam Z. Higgins Freeze-drying, or lyophilization, has shown great promise in addressing many of the logistical challenges of storing and preserving red blood cells (RBCs). A crucial part of any future RBC lyophilization protocol is the primary drying temperature, which affects the sample drying rate and the dried cake’s abil...

Protein-coated magnetic nanoparticles are promising candidates for various medical applications. Prior to their application into a biological system, one has to guarantee that the particle dispersions are free from pathogens or any other microbiologic contamination. Furthermore, to find entrance into clinical routine, the nanoparticle dispersions have to be storable for several months. In this ...

Viral safety is a prerequisite for manufacturing clinical antihemophilic factor VIII concentrates from human plasma. With particular regard to the hepatitis A virus (HAV), a terminal dry-heat treatment (100 degrees for 30 min) process, following lyophilization, was developed to improve the virus safety of a solvent/detergent-treated antihemophilic factor VIII concentrate. The loss of factor VII...

HYPOTHESIS Lyophilized plasma (LP) is as safe and effective as fresh frozen plasma (FFP) for resuscitation after severe trauma. DESIGN Multicenter animal study. SETTING Animal laboratories, 2 level I trauma centers. PARTICIPANTS Thirty-two Yorkshire crossbred swine. INTERVENTIONS Lyophilized plasma was analyzed for factor levels and clotting activity before lyophilization and after reco...

The aim of this research was to prepare the inclusion complexes formed by hesperidin and (2-hydroxypropyl)-β-cyclodextrin by different techniques (kneading, co-evaporation and lyophilization methods), to carry out phase solubility studies, in vitro dissolution, antioxidant and antimicrobial tests. To confirm the inclusion compounds formation, spectroscopic (UV-Vis, FTIR, 1 H-NMR) and thermal (D...

The necessity of measuring permeability is encountered in number applications and some them have adequate measurement techniques available. However, the fields freeze drying, composite scaffolds, 3D printed materials, etc., sample materials unique shapes sizes, which disables use standardized equipment. Hence, a novel method was developed to determine material by analyzing high frequency unstea...