Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...

Synchronization of rumen available protein and energy is one of the conceptual methods to increase the efficiency of utilization of nutrients by the ruminants The concept of synchronization energy and protein was first by Jhonson, Implying that maximum microbial protein synthesis could be achieved by matching the rate of Organic Matter and protein degradation. Synchronization of rumen available...

abstract ethanol has a vast consumption around the world. many researches confirmed some adverse effect of this component on human health. in addition, recent studies showed significant alteration in both cellular population, and protein profile of human foreskin fibroblast cell line (hfff2) in the specific dosage of ethanol. here, the role and interaction of some proteins (characterized by sig...

lyme borreliosis is a tick-born disease caused by the spirochete borrelia burgdorferi. the aim of this research was molecular typing of different strains of b. burgdorferi and protective efficacy of outer surface protein in animal model. seven strains of b. burgdorferi isolated from skin and csf of lyme disease patients were compared using pcr- rflp as well as dna and amino acid sequences. the ...

objective(s) staphylococcus aureus is a foremost source of numerous nosocomial and community acquired infections. antibiotic therapy for vancomycin resistant s. aureus (vrsa) can not promise the eradication of infections. since adhesion is the major route of infections, adhesin based vaccine could suppress s. aureus infections. fibronectin binding protein a (fnbpa) and clumping factor a (clfa) ...

recombinant human interferon alpha 2a (rhifn α -2a) production and cell growth were monitored in a set of genetically modified e. coli strains (msd1519, msd1520, msd 1521, msd 1522, msd 1523) producing rhifn α -2a. the growth was followed at od 600 nm, changes in cell physiology were detected by pyrolysis mass spectrometry (pyms) of cell biomass and recombinant protein production was determined...