A simplified method for the analysis of both the glyceride-rich Sf 20-1 O5 and the cholesterol-rich St 0-20 lowdensity lipoproteins is presented. It consists of serum lipoprotein fractionation by preparative ultracentrifugation and subsequent quantitative analysis by refractometry. Comparison of this technique with the technically more difficult analytic ultracentrifugal methodology reveals com...

Obesity in children has also been associated with the development of early cardiovascular abnormalities. The aim of this study was to investigate the relationship between aortic stiffness and some risk factors in obese children. Sixty obese children and 60 age- and sex-matched healthy controls were assessed. The mechanical property parameters were measured or computed: lumen diastolic and systo...

The A-type lamin deficient mouse line (Lmna (-/-) ) has become one of the most frequently used models for providing insights into many different aspects of A-type lamin function. To elucidate the function of Lmna in the growth and metabolism of mice, tissue growth and blood biochemistry were monitored in Lmna-deficient mice, heterozygous (Lmna (+/-) ) and wide-type (Lmna (+) (/) (+) ) backcross...

Many clinical studies have shown an increased insulin response to oral glucose in patients with ischemia of the heart, lower limbs, or brain. Hyperinsulinemia also occurs in patients with angiographically proved atherosclerosis without ischemia and thus appears to be related to arterial disease and not to be a nonspecific response to tissue injury. Fasting insulin levels and insulin responses t...

The excessive synthesis and storage of triacylglycerol (triglyceride) has a number of clinical implications. It is obviously the main symptom of obesity where excess lipid is stored in adipose tissue. Abnormal accumulations of triacylglycerols can also be manifested as a fatty liver, e.g. as a'result of the ingestion or inhalation of toxic compounds and damage to the liver. These conditions are...

BACKGROUND In patients with discordance between low-density lipoprotein (LDL) cholesterol and LDL particle (LDL-P) concentrations, cardiovascular risk more closely correlates with LDL-P. METHODS AND RESULTS We investigated the effect of alirocumab, a fully human monoclonal antibody to proprotein convertase subtilisin/kexin type 9, on lipoprotein particle concentration and size in hypercholest...

Epidemiological and clinical studies have consistently demonstrated that increased concentrations of LDL cholesterol in plasma are associated with increased risk of atherosclerotic cardiovascular disease (CVD). The reference method for LDL cholesterol is -quantification, a method developed by the CDC that requires ultracentrifugation and is accordingly labor intensive, time consuming, and expen...

Lovastatin and simvastatin which are very potent cellular cholesterol biosynthesis inhibitors, significantly affect the plasma lipoprotein concentration. After incubation of plasma with 14C-labelled compounds, radioactivity was found in all lipoprotein fractions but mainly (40%) in high density lipoprotein (HDL), and in the lipoprotein-deficient plasma fraction (20-30%). Drug-treated lipoprotei...

the non-enzymatic carbamylation of low density lipoprotein (ldl) is a naturally occurring chemical modification of apolipoprotein b as a result of condensation between lysine residues and cyanate derived from urea. carbamylated ldl is poorly recognized by ldl receptors and initiates different processes that can be considered proatherogenic. thus, ldl carbamylation may contribute to the increase...

the oxidative modification of low density lipoprotein (ldl) may play an important role in atherogenesis. antioxidants that can prevent ldl oxidation may act as antiatherogens. our understanding of the mechanism of ldl oxidation and factors that determine its susceptibility to oxidation is still incomplete. copper is a candidate for oxidizing ldl in atherosclerotic lesions. the binding of copper...