Acyl-CoA synthetases play a pivotal role in fatty acid metabolism, providing activated substrates for fatty acid catabolic and anabolic pathways. Acyl-CoA synthetases comprise numerous proteins with diverse substrate specificities, tissue expression patterns, and subcellular localizations, suggesting that each enzyme directs fatty acids toward a specific metabolic fate. We reported that hBG1, t...

Source/description: Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an essential enzyme in fatty acid metabolism. It converts free long-chain fatty acids into fatty acylCoA esters, which are key intermediates in the synthesis of complex lipids. ACSL4 catalyses the formation of these fatty acyl-CoAs by a two-step process that starts with the addition of an AMP at the carboxyl group of ...

Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase (Nmt1p) is a monomeric enzyme that is essential for vegetative growth. Nmt1p catalyzes the co-translational transfer of myristate from CoA to the amino-terminal Gly of cellular proteins in an ordered Bi Bi reaction mechanism that initially involves binding of myristoyl-CoA to the apoenzyme. Forty one fatty acid analogs were s...

Cells of the yeast sterol auxotroph GL7 were grown on either ergosterol or cholesterol to mid-logarithmic phase and total membrane fractions prepared. Activities of phospholipid biosynthetic enzymes in the two cell types were determined. The rates of phosphatidyl-ethanolamine-phosphatidyl-choline-N-methyl transferase and acyl-CoA-alpha-glycerol-3-phosphate transcylase were significantly greater...

Fatty acid transporter proteins (FATPs) are a family (SLC27) of six transporters (FATP1-6). They have at least one, and possibly [6, 13], transmembrane segments, predicted on the basis structural similarities to form dimers. SLC27 members several domains: integral membrane associated domain, peripheral FATP signature, intracellular AMP binding motif, dimerization lipocalin an ER localization do...

A yeast system differs only in that the major product is stearylCo.4 (5, 6) rather than the free fatty acid, palmitate, as in the other systems. The fatty acid synthetase reaction appears to be a multistage reaction catalyzed by a particulate enzyme complex estimated to have a molecular weight of 2,000,OOO in yeast (6) and to be readily sedimentable by ultracentrifugation in adipose tissue (4) ...

Submit Manuscript | http://medcraveonline.com Abbreviations: PUFA: Polyunsaturated Fatty Acid; PCR: Polymerase Chain Reaction; DNA: Deoxyribonucleic Acid; RNA: Ribonucleic Acid; SFA: Saturated Fatty Acid; MUFA: Monounsaturated Fatty Acid; SCD: Δ9-desaturase; FASN: Fatty Acids Synthase; STAT5: Signal Transducer and Activator of Transcription 5; CEBPA: CCAAT/Enhancer-Binding protein (alpha); CLA:...

A yeast system differs only in that the major product is stearylCo.4 (5, 6) rather than the free fatty acid, palmitate, as in the other systems. The fatty acid synthetase reaction appears to be a multistage reaction catalyzed by a particulate enzyme complex estimated to have a molecular weight of 2,000,OOO in yeast (6) and to be readily sedimentable by ultracentrifugation in adipose tissue (4) ...

It is now well established that the peroxisomal membrane is not freely permeable to small molecules in vivo, which implies the existence of metabolite transporters in the peroxisomal membrane. A few putative peroxisomal metabolite transporters have indeed been identified, but the function of these proteins has remained largely unresolved so far. The only peroxisomal transporter characterized to...

Three straight chain acyl-CoA dehydrogenases were purified to apparent homogeneity from bovine liver using 40-70% (NH4)2SO4 precipitation, gel filtration, DEAE-cellulose column chromatography, and preparative electrophoresis. Separation of the acyl-CoA dehydrogenases by these procedures has been efficiently monitored by two newly developed analytical methods: (i) native staining of acyl-CoA deh...