Sliding clamps are ring-shaped proteins that tether DNA polymerases to their templates during processive DNA replication. The action of ATP-dependent clamp loader complexes is required to open the circular clamps and to load them onto DNA. The crystal structure of the pentameric clamp loader complex from Escherichia coli (the gamma complex), determined in the absence of nucleotides, revealed a ...

Optimal control of a wheel loader operating in the short loading cycle is studied in order to investigate the potentials for fuel consumption reduction while maintaining acceptable production rates. The wheel loader is modeled as a system with five states and three control inputs including torque converter nonlinearities. The torque converter is modeled with no lockup enabling power transmissio...

Authors have been conducting research on an autonomous system for loading operation by wheel loader. In loading operation with V shape loading method, a time for travelling is the dominant factor for the cycle time. The path should be modified varied condition of the working place. To achieve quicker and reliable operation in autonomous loading operation, new path planning method is developed. ...

Object-oriented applications execute by performing operations on objects. In suites of inter-operating or integrated applications, such as are essential in an enterprise setting, the same objects must be manipulated by multiple applications. Each application generally requires its own particular operations to be supported. For example, a Shipping application might require Truck objects to suppo...

Processive chromosomal replication relies on sliding DNA clamps, which are loaded onto DNA by pentameric clamp loader complexes belonging to the AAA+ family of adenosine triphosphatases (ATPases). We present structures for the ATP-bound state of the clamp loader complex from bacteriophage T4, bound to an open clamp and primer-template DNA. The clamp loader traps a spiral conformation of the ope...

The wheel loader is modeled as a mechanical manipulator with four degrees of freedom (DOFs), which comprises a seven-bar linkage using the symbolic notation of Denavit and Hartenberg. Homogeneous transformation matrices are used to capture the spatial configuration between adjacent links. The forward kinematics method is used to formulate the kinematics equations by attaching Cartesian coordina...

Clamp loaders load sliding clamps onto primer-template DNA. The structure of the E. coli clamp loader bound to DNA reveals the formation of an ATP-dependent spiral of ATPase domains that tracks only the template strand, allowing recognition of both RNA and DNA primers. Unlike hexameric helicases, in which DNA translocation requires distinct conformations of the ATPase domains, the clamp loader ...

Replication machines use ring-shaped clamps that encircle DNA to tether the polymerase to the chromosome. The clamp is assembled on DNA by a clamp loader. This report shows that the polymerase and clamp loader coordinate their actions with the clamp by competing for it through overlapping binding sites. The competition is modulated by DNA. In the absence of DNA, the clamp associates with the cl...