Objective(s) Phosphatidate phosphohydrolase (PAP) catalyzes the dephosphorylation of phosphatidic acid to yield Pi and  diacylglycerol. Two different forms of PAP in rat hepatocyte have been reported. PAP1 is located in cytosolic and microsomal fractions and participates in the synthesis of triacylglycerols, phosphatidylcholine, and phosphatidylethanolamine, whereas the other form of phosphati...

Pork liver contains an endogenous enzyme, ferrochelatase (FeCH), which catalyses the formation of zinc protoporphyrin (ZnPP), a natural pigment great interest for meat industry. The aim this study was to analyse effect pork drying (from −10 70 °C), as stabilisation method, on FeCH activity (EA) and apparent concentration (EC app ). Drying temperatures close room conditions 10 20 °C) allowed pre...

Glutamate dehydrogenase has been purified and crystallized from rat liver mitochondria and from whole rat liver preparations. No differences in the enzyme prepared by the two different methods have been observed and there is no evidence that more than one glutamate dehydrogenase occurs in rat liver. The pure enzyme has a molecular weight of 350,000 f 20,000 and consists of six to eight subunits...

Background: Recently, applications of nanoparticles in many fields of medicine have been developed, due to their specific physical and chemical properties. Therefore assessment of their toxicity specially in the in vivo condition is necessary. Objectives: The aim of this study is to evaluation the effect of Fe3O4 nanoparticles coating by biocompatible compounds on their t...

the incidence of drug induced liver injury (dili) is about 1/1000 to 1/10000 among patients who receive therapeutic drug doses. drug induced hepatotoxicity is a major cause of acute and chronic liver disease. the severity of liver damage ranges from nonspecific changes in liver structure to acute liver failure, cirrhosis and liver cancer. some common agents that can cause liver injury are aceta...

background salep is used for various purposes in food industries and traditional medicine. therefore, evaluation of its effect on the liver seems to be necessary. objectives the aim of this study was to assess salep effect on liver. materials and methods in this experimental study, various concentrations of salep were intraperitoneally administered to five groups of wistar rats (control, placeb...

A newly developed specific radioimmunoassay was used to quantify phosphofructokinase protein directly and independently of assayable activity in liver and kidney cytosol of normal fed, starved and alloxan-diabetic rats. In the fed state, liver phosphofructokinase concentration was 0.096 microM and the kidney enzyme was 0.086 microM (mumol/kg of tissue). In the starved state (24h), liver and kid...

background nonalcoholic steatohepatitis (nash) is a common liver disease that can progress to cirrhosis or hepatocellular carcinoma. it is estimated that up to 3% of the iranian population have this condition. although the pathogenesis of nash is incompletely understood, there is significant evidence pointing to the importance of insulin resistance. metformin is an oral hypoglycemic agent known...

activities of the key enzymes of gluconeogenesis in the liver and kidney of the foetal guinea pig have been followed. The foetal guinea pigs used had a gestational age of 68-72 days. Pyruvate carboxylase was present at very low activities in the kidney of the foetus and neonatal animal and also in the foetal liver until day 50, when it increased in activity to reach a value by day 60 of more th...

the effects of therapeutic doses of dillsun, garsin, antum and statins on rat liver cytosolic phosphatidate phosphohydrolase (pap) activity, a key enzyme in triacylglycerol synthesis, and on serum and liver lipids were examined. lovastatin and simvastatin both stimulated the enzyme activity by 29% and 43%, respectively. the stimulatory effects were dose-dependent and accompanied by the decline ...