Immunity against infection with Listeria monocytogenes is not achieved from innate immune stimulation by contact with killed but requires viable Listeria gaining access to the cytosol of infected cells. It has remained ill-defined how such immune sensing of live Listeria occurs. Here, we report that efficient cytosolic immune sensing requires access of nucleic acids derived from live Listeria t...

Immunization of mice with nonviable Listeria monocytogenes generates an insufficient CD8(+) T cell response and consequently only limited protection against subsequent L. monocytogenes infection. We have recently demonstrated that depletion of regulatory CD4(+) T cells during immunization significantly enhances CD8(+) T cell responses. In the present study, we determined the impact of CD4(+) T ...

Listeriosis in human is clinically characterized as perinatal listeriosis, neonatal listeriosis and adult listeriosis. Listeria spp are an established pathogen in animals, and human infections are usually seen following ingestion of contaminated food (processed food). Human infections of Listeria spp initially present with gastrointestinal symptoms. Meningitis, septicaemia, brain abscess and pr...

discussion l. monocytogenes can cause progressive and rapidly fatal infections in the vulnerable persons, with multisystem involvement. since this bacterium is not susceptible to cephalosporines, it will be better to consider effective antimicrobials in the treatment of the possible cases. case presentation a 70-years-old patient with chronic renal failure was admitted to the infectious disease...

in this study, the effects of various concentrations of nacl and kcl and partial replacement of nacl withkcl on the growth characteristics of l. monocytogenes were evaluated. it was found that l. monocytogenescan grow in the presence of 1-9% nacl and 1-11% kcl. the higher the concentration of salt used, the longerthe lag phase induced. in addition, it was observed that l. monocytogenes tolerate...

conclusions l. innocua is not a pathogen, but the presence of the bacterium could be an indicator of probable contamination with l. monocytogenes. moreover, there is a potential risk to public health from the consumption of raw or undercooked burgers, which may increase the possibility of the acquisition of resistance to antibiotics. objectives the aim of this study was to evaluate the occurren...

The objective of this study was to isolation and characterization of Listeria species and determines Listeria monocytogenes serotypes in fresh fish, shrimp, crab and lobster in Isfahan and Shahrekord, Iran. From September 2010 to April 2011, a total of 300 samples of fresh fish, shrimp, crab and lobster were obtained from randomly selected retail stores in Isfahan and Shahreko...

Mitochondria are essential and highly dynamic organelles, constantly undergoing fusion and fission. We analyzed mitochondrial dynamics during infection with the human bacterial pathogen Listeria monocytogenes and show that this infection profoundly alters mitochondrial dynamics by causing transient mitochondrial network fragmentation. Mitochondrial fragmentation is specific to pathogenic Lister...

Listeria can escape host autophagy defense pathways through mechanisms that remain poorly understood. We show here that in epithelial cells, Listeriolysin (LLO)-dependent cytosolic escape of Listeria triggered a transient amino-acid starvation host response characterized by GCN2 phosphorylation, ATF3 induction and mTOR inhibition, the latter favouring a pro-autophagic cellular environment. Surp...

Subclinical infection of BALB/c mice with the intracellular bacterial pathogen Listeria monocytogenes results in the development of protective antilisterial immunity. L. monocytogenes can infect hepatocytes, and antilisterial cytotoxic T lymphocytes (CTL) lyse Listeria-infected hepatocytes in a major histocompatibility complex (MHC) class Ia-restricted manner. It remained to be determined wheth...