Hispidin is an important styrylpyrone produced by Sanghuangporus sanghuang. To analyze hispidin biosynthesis in S. sanghuang, the transcriptomes of hispidin-producing and non-producing sanghuang were determined Illumina sequencing. Five PKSs identified using genome annotation. Comparative analysis with reference transcriptome showed that two (ShPKS3 ShPKS4) had low expression levels four types ...

in previous studies, mebudipine, a dihydropyridine calcium channel blocker, showed a considerable potential to be used in cardiovascular diseases. the aim of the current study was to develop a valid method using reversed-phase high performance liquid chromatography coupled with electrospray ionization mass spectrometry to assay mebudipine in the human plasma. separation was achieved on a zorbax...

Donepezil is used worldwide for the treatment of Alzheimer’s disease, where it is used to increase cortical acetylcholine. We present a simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method developed and validated for the quantification of Donepezil in Human Plasma. Quetiapine was used as the internal standard. The simple and cost effective Liquid-Liquid Extraction method is a...

Project Goals: The project is to develop correlated optical (Raman and SH-OCT) and mass spectrometric (SIMS and MALDI MS) imaging approaches for spatial and temporal characterization of lignocellulosic materials at specific processing stages. The ability to efficiently use lignocellulosic materials (LCMs) to feed the biorefinery of the future depends on high-efficiency pre-enzymatic processing ...

In this study, centrifugal ultrafiltration coupled with high performance liquid chromatographymass spectrometry was utilized to screen and identify xanthine oxidase inhibitors from Puerariae flos extract. The experimental conditions of centrifugal ultrafiltration including xanthine oxidase concentration, incubation time, pH and temperature were optimized. At the optimum condition (xanthine oxid...

As analytical and bioanalytical methods must be validated before using them for routine sample analysis and after changing method parameters (see Chapter 8), instruments such as liquid chromatography coupled with mass spectrometry (LCMS) or tandem mass spectrometry (LC-MS/MS), which are utilized to perform the analysis, should be calibrated and qualified. In addition, an instrument’s performanc...

Atopic dermatitis (AD) is a widely prevalent and chronically relapsing inflammatory skin disease. Penta Herbs Formula (PHF) is efficacious in improving the quality of life and reducing topical corticosteroid used in children with AD and one of the active herbs it contains is Cortex Moutan. Recent studies showed that altered functions of dendritic cells (DC) were observed in atopic individuals, ...

in this work, a procedure for preconcentration of cobalt using dispersive liquid–liquid microextraction (dllme) with the reagent 1-nitroso 2-naphtol as complexing reagent was developed. at ph 4, co(ii) was complexed with 1-nitroso 2-naphtol and extracted into the fine droplets formed when mixing chloroform (extraction solvent), ethanol (disperser solvent) and the sample solution. after extracti...

in this work, a procedure for preconcentration of silver using dispersive liquid–liquid microextraction with the reagent dithizone as complexing reagent was developed. at ph 2, silver ions are complexed with dithizone and extracted into the fine droplets formed when mixing carbon tetrachloride (extraction solvent), ethanol (disperser solvent) and the sample solution. after extraction, the phase...

thallium is widely found in nature, but the only inorganic stones full of this element are crookesite and lorandit. it isalso found in pyrites of copper, lead and inorganic stones. the element and its compositions are toxic and harmful to theenvironment; therefore, its application requires caution and further research. it is important to develop sensitive andaccurate analytical methods to deter...