Very low density lipoproteins (VLDL) and low density lipoproteins (LDL) from human normolipemic plasma, and the VLDL, the intermediate density lipoprotein (IDL), and LDL from patients with Type III hyperlipoproteinemic plasma were tested for their abilities to suppress the activity of 3-hydroxy-3-methylglutaryl-Coenzyme A (HMG-CoA) reductase in cultured human fibroblasts from normal subjects an...

Previously, we demonstrated in cultured dorsal root ganglion neurons that, in the presence of beta-migrating very low density lipoproteins (beta-VLDL), apolipoprotein (apo) E4, but not apoE3, suppresses neurite outgrowth. In the current studies, murine neuroblastoma cells (Neuro-2a) were stably transfected with human apoE3 or apoE4 cDNA, and the effect on neurite outgrowth was examined. The sta...

Very low density lipoproteins (VLDL), Sf60 to 400, from normolipemic individuals do not suppress 3-hydroxy-3-methylglutaryl-CoA reductase activity in cultured normal human fibroblasts at concentrations 20fold higher than those of low density lipoproteins (LDL) that give total suppression. To determine if these VLDL contain all of the structural elements necessary for receptor-mediated suppressi...

The 12 laboratories of the North American Lipid Research Clinics performed lipid and lipoprotein analyses on aliquots of 53 freshly prepared plasma samples during four years. The samples, from normal, mildly hyperlipidemic, and treated hyperlipidemic subjects, were analyzed for total cholesterol, triglycerides, and cholesterol in verylow-density lipoproteins (VLDL), low-density lipoproteins (LD...

To quantify more precisely the metabolism of apolipoprotein B (apo B) in human beings, an integrated model was developed for the analysis of the isotope kinetics of apo B in very low density lipoproteins (VLDL), intermediate density lipoproteins (IDL), and low density lipoproteins (LDL). The experimental basis for model development was a series of 30 triple-isotope studies in which patients rec...

Subfractions of radioiodinated d less than 1.019 g/ml lipoproteins were isolated by nonequilibrium density gradient ultracentrifugation (DGU) from normal and dysbetalipoproteinemic human plasma and were injected into rats. Size and density (d) of lipoprotein products formed over 8 hours were assessed by gradient gel electrophoresis and equilibrium DGU, respectively. Subfractions containing a su...

A study was undertaken to determine the relative association of lipid and apolipoproteins among lipoproteins produced during lipolysis of very low density lipoproteins (VLDL) in perfused rat heart. Human VLDL was perfused through beating rat hearts along with various combinations of albumin (0.5%), HDL2, the infranatant of d greater than 1.08 g/ml of serum, and labeled sucrose. The products wer...

although there are many evidences regard to the relationship between serum thyroid hormones, cholesterol, triglyceride and lipoproteins in different animals, but there are limited references in dogs. the aim of this study was to determine the correlation between thyroid hormones and concentrations of serum lipid and lipoprotein profiles, and also to detect relationship between the measured para...

Chylomicrons and very low density lipoproteins (VLDL) are produced by the intestine and these nascent particles are thought to be similar to their counterparts in intestinal lymph. To study the relationship between these lipoproteins within the cell and those secreted into the lamina propria and lymph, we have isolated enterocytes, lamina propria, and mesenteric lymph from rats while fasted and...

Animals fed cholesterol accumulate several types of cholesterol-rich lipoproteins in their plasma and ultimately develop cholesteryl ester deposition in tissue macrophages. Previous studies in the cholesterol-fed dog have shown that one class of cholesterol-rich lipoproteins, P-migrating very low density lipoproteins (P-VLDI,, density < 1.006 g/ml), possesses a unique ability to produce cellula...