background: the homologous recombination (hr) is one of the conventional cloning methods for the production of recombinant dna. it is a quick method for in vivo dna cloning without using the high cost restriction enzymes. a few modifications in the cloning procedure can increase the efficiency of this method. m aterials and methods: in this study, effect of heating on the rate of the igg1 heavy...

a new system is presented for the generation ofrecombinant bacillus subtilis strains without antibioticmarkers. this system is based on two plasmids constructed in escherichia coli. the first plasmid phm30contains an incomplete hisi gene, the last gene in thehistidine biosynthesis operon of b. subtilis and part ofthe genes yvca and yvcb of unkown function flankinghisi at the 3´-end. the spectin...

toxoplasma gondii contain various immunogenic antigens. the most important toxoplasma antigens are somatic and excreted/secreted antigens. rhoptry proteins are known as excreted/secreted antigens. these antigens have been proposed as a vaccine candidate against toxoplasmosis. the main objective of the present work was cloning rhoptry protein1 (rop1) gene of toxoplasma gondii (rh) in a cloning v...

The thymidylate synthetase gene of B. subtilis bacteriophage Phi-3-T, when cloned in plasmids pSC101 or pMB9 is expressed in E. coli. The promoter of the cloned gene is likely to originate in Phi-3-T. Rearrangements of hybrid plasmid sequences during the cloning have been noted. B. subtilis strains can be transformed with hybrid DNAs. The transformants contain sequences of Phi-3-T, but not thos...

background: serological assay based on dense granular (gra) proteins of toxoplasma gondii (t. gondii) is actually the most popular laboratory diagnostic tool to detection of toxoplasmosis. we aimed to construct a recombinant gra7-ptz57rt plasmid vectors that it is suitable for sub-cloning and gra7 protein production. m aterials and methods: souris mice were used for maintaining of t. gondii tac...

Coronavirus disease (COVID-19) causes a serious threat to human health. Virus-like particles (VLPs) constitute promising platform in SARS-CoV-2 vaccine development. In this study, the E, M, and S genes were cloned into multiple cloning sites of new triple expression plasmid with one p10 promoter, two pPH promoters, three sites. The was transformed DH10 BacTMEscherichia coli competent cells obta...

Quorum sensing is a mechanism for regulating proteobacterial gene expression in response to changes in cell population. In proteobacteria, N-acyl homoserine lactone (AHL) appears to be the most widely used signalling molecules in mediating, among others, the production of extracellular virulence factors for survival. In this work, the genome of B. cepacia strain GG4, a plasmid-free strain capab...

conclusions the results of the present study will increase our knowledge about molecular cloning and expression of the l. infantum kmp-11 gene, and this may be used as an effective target for controlling visceral leishmaniasis. results the kmp-11 gene was successfully subcloned in pcdna3 as a eukaryotic expression vector. recombinant kmp-11 protein was confirmed by the reverse transcriptase pol...

objective: in this study, two conserved genes (m1 and np) of influenza virus were expressed in a bicistronic vector in order to develop a universal gene based vaccine. materials and methods: plasmids m1-pires2-egfp, pires2-np were constructed by cloning the pcr products of m1 and np genes which were amplified from the a/peurto rico/8/34 (h1n1) influenza virus strain into the plasmid expression...