نتایج جستجو برای: linear epitope
تعداد نتایج: 500550 فیلتر نتایج به سال:
Epitope-based design of vaccines, immunotherapeutics, and immunodiagnostics is complicated by structural changes that radically alter immunological outcomes. This is obscured by expressing redundancy among linear-epitope data as fractional sequence-alignment identity, which fails to account for potentially drastic loss of binding affinity due to single-residue substitutions even where these mig...
The plethora of virulence factors associated with Staphylococcus aureus make this bacterium an attractive candidate for a molecularly-designed epitope-focused vaccine. This approach, which necessitates the identification of neutralizing epitopes for incorporation into a vaccine construct, is being evaluated for pathogens where conventional approaches have failed to elicit protective humoral res...
Immunogenic C. coli CjaA protein is a candidate for a chicken anti-Campylobacter subunit vaccine. In order to enhance its immunogenicity an antigenic determinant of the CjaA protein was identified. Thereafter, two copies of the antigenic epitope were cloned in tandem directly or with a flexible hinge between them. All experiments documented that the CjaA protein contained not only linear antige...
Antibodies empower numerous important scientific, clinical, diagnostic, and industrial applications. Ideally, the epitope(s) targeted by an antibody should be identified and characterized, thereby establishing antibody reactivity, highlighting possible cross-reactivities, and perhaps even warning against unwanted (e.g. autoimmune) reactivities. Antibodies target proteins as either conformationa...
Rational design of peptide vaccines becomes important for the treatment of some diseases such as Alzheimer's disease (AD) and related disorders. In this study, as part of a larger effort to explore correlations of structure and activity, we attempt to characterize the doubly phosphorylated chimeric peptide vaccine targeting a hyperphosphorylated epitope of the Tau protein. The 28-mer linear chi...
We describe a general synthetic strategy for developing high-affinity peptide binders against specific epitopes of challenging protein biomarkers. The epitope of interest is synthesized as a polypeptide, with a detection biotin tag and a strategically placed azide (or alkyne) presenting amino acid. This synthetic epitope (SynEp) is incubated with a library of complementary alkyne or azide prese...
234 words 24 Abstract (max 250 words, currently 234) 27 Hepatitis C virus (HCV) E2 envelope glycoprotein is crucial for virus entry into hepatocytes. A conserved 28 region of E2 encompassing amino acids 412-423 (epitope I) and containing Trp420, a residue critical for 29 virus entry, is recognized by several broadly neutralizing antibodies. Peptides embodying this epitope I 30 sequence adopt a ...
Monoclonal antibodies have proved powerful tools in serological techniques and as probes for elucidating the antigenic determinants of macromolecules and cells. However an inherent problem of these reagents is the phenomenon of assay restriction, where a given antibody may perform well in some assays, whilst being poor or ineffective in others [I] . Thus stringent evaluation of a monoclonal ant...
This study describes the identification of the epitope recognized by the tobacco mosaic virus (TMV) coat protein (CP)-specific monoclonal antibody 29 (MAb29) by displaying a CP gene-fragment library on pVIII of filamentous phage M13. More than 80% of the clones isolated after one round of panning bound specifically to MAb29. DNA sequencing of ten randomly chosen MAb29-specific clones and subseq...
One of the major challenges in designing a peptide-based vaccine is the identification of antigenic regions in an antigen that can stimulate B-cell's response, also called B-cell epitopes. In the past, several methods have been developed for the prediction of conformational and linear (or continuous) B-cell epitopes. However, the existing methods for predicting linear B-cell epitopes are far fr...
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