We report the first case of an immunocompromised adult patient presenting with cervicofacial lymphadenitis due to Mycobacterium haemophilum, confirmed using hsp65 gene sequencing and line-probe assays. In resource-limited settings, especially in developing countries, appropriate culture methods and rapid molecular diagnostic tools such as hsp65 gene sequencing for identification of this organis...

Infections with multidrug-resistant (resistant at least to rifampicin, RIF and isoniazid, INH) strains of Mycobacterium tuberculosis (MDR-TB) are associated with high case fatality rates. Rapid identification' of MDR-TB strains is important for early institution of appropriate therapy. Two DNA line probe assays, GenoType MTBDR (GT-MTBDR) and INNO-LiPA Rif. TB (INNO-LiPA) were compared for their...

Two methods for genotyping hepatitis C virus (DNA enzyme immunoassay [DEIA] and line probe assay [Inno-LiPA HCV I and II]) were compared on 120 samples and of these 87% were assigned to the same subtype by both assays. There were 15 subtyping discrepancies which involved 5% of type 1 isolates and 90% of type 2 isolates. Amplified products from the core and 5' untranslated regions (UTR) were seq...

Histone acetyltransferases (HATs) are a class of epigenetic enzymes involved in critical cellular processes like nucleosome assembly, DNA damage repair, and transcriptional regulation. HATs are implicated in many human pathologies including cancers. This chapter describes essential experimental considerations for performing high-throughput screening assays, and offers practical strategies for a...

We develop a triple-color fluorescent probe which may function as a lab-on-a-DNA-molecule for simultaneous detection of multiple exonucleases/restriction endonucleases. This triple-color fluorescent probe can be further applied for the discrimination of seven exonucleases and four cell lines as well as the screening of various nuclease inhibitors.

the objective of the study was to evaluate thecytotoxic effects of aqueous fruit extract of cucumis melo in human prostate cancer cell line (pc-3) using mttand neutral red assays. the crude aqueous extract of c.melo was prepared by cold maceration method, filtered,concentrated and tested on pc-3 cell line. dose-dependent cytotoxic activitieswere exhibited by human prostate carcinoma pc-3 cell l...

I describe the use of latex agglutination as a simple readout for DNA hybridization assays. Latex agglutina-tion is a well-known readout for antibody-based diagnos-tics (1, 2) and uses " sandwich " assay chemistry for which analogous DNA assays are well known (3, 4). Latex agglutination is extremely simple and chemically robust, and latex particles have been used as a label in DNA hybridization...

Assessment of the cellular effects of natural and/or synthetic products is one of the major goals in biological experiments. This assessment may be conducted at different levels; cellular viability, cellular biological activity, and cellular structure and morphology. Different assays are available for any of above purposes. It is becoming of a routine exercise to apply any of these assays, and ...

BACKGROUND Real-time assays for Yellow fever virus (YFV) would help to improve acute diagnostics in outbreak investigations. OBJECTIVES To develop a real-time assay for YFV able to detect African and South American strains. STUDY DESIGN Three short probe (14-18 nt) formats were compared and a plasmid-transcribed RNA standard was used to test the performance of the assays. Additionally the n...