Nowadays, the discovery of lignocellulolytic microorganisms that are better adapted to operational conditions while exhibiting the strong degrading activities is highly desired for successful lignocellulose biotransformation processes. In this study, microorganisms were isolated from lignocellulose-rich composting materials by selective methods. A screening of isolates known to have lignocellul...

The use of residual lignocellulosic biomass appears as an opportunity to obtain high added value products like enzymes. present work goal was select basidiomycetes capable metabolizing empty fruit bunches (EB) or sludge decanter (SD) from the oil palm industry produce enzymatic cocktails. First, eight macro-basidiomycetes strains Brazilian cerrado were selected based on genomic DNA analyses out...

The genomes of three representative Polyporales (Bjerkandera adusta, Phlebia brevispora and a member of the Ganoderma lucidum complex) were sequenced to expand our knowledge on the diversity of ligninolytic and related peroxidase genes in this Basidiomycota order that includes most wood-rotting fungi. The survey was completed by analyzing the heme-peroxidase genes in the already available genom...

BACKGROUND Total three Pleurotus species (P. ostreatus, P. sapidus, P. florida) was compared for ligninolytic enzyme production grown with Coralene Golden Yellow, Coralene Navy Blue and Coralene Dark Red azo dyes in liquid medium under shaking condition. RESULTS The biodegradation competency varied from species to species and it was found that P. ostreatus, P. sapidus and P. florida to 20 ppm...

We identified a highly expressed protein (BUNA2) by two-dimensional gel electrophoresis from the hyper lignin-degrading fungus Phanerochaete sordida YK-624 under wood-rotting conditions. Partial amino acid sequences of BUNA2 were determined by LC-MS/MS analysis, and BUNA2 gene (bee2) and promoter region were PCR-cloned and sequenced. The bee2 promoter was used to drive the expression of the man...

Phanerochaete chrysosporium is a white rot fungus which secretes a family of lignin-degrading enzymes under nutrient limitation. PSBL-1 is a mutant of this organism that generates the ligninolytic system under nonlimiting conditions during primary metabolism. Lignin peroxidase, manganese peroxidase, and glyoxal oxidase activities for PSBL-1 under nonlimiting conditions were 4- to 10-fold higher...

Trametes hirsuta is an efficient lignin-degrading species due to its ability to produce laccase and Mn-dependent peroxidase. Agricultural residues represent prospective substrates for the bioconversion into fungal biomass and lignocellulolytic enzymes, but also they could be potential environmental pollutants. Evaluation of the potential of T. hirsuta to produce ligninolytic enzymes during soli...

The white-rot fungi produce wide range of extracellular enzymes (especially ligninolytic enzymes) and they can degrade complicated and difficult degradable compounds. An immobilization of enzymes causes their stability, reusability and cheapness, so they could be effectively used in biotechnologies and other branches. In this work we focused on the optimization of isolation procedure namely pre...