Objectives: The bioactive phytocompounds present in Zingiber officinale were assessed for their tumor-suppressing activity against TP-53 BP1 linked to hepatocellular carcinoma. Methods: study investigates the interaction of phytochemicals from Ginger (Zingiber officinale) with tumor protein TP53. Drug likeness chosen ligand was evaluated using SWISS ADME. Autodock tools used investigate interac...

there are two different co-expression systems including bicistronic; dual-vector or two-promoter to express two different genes simultaneously and also to study protein-protein interactions. bicistronic system has disadvantages e.g. compared with two-promoter system. in this paper, a simple method based on spliced overlap extension by polymerase chain reaction (soe-pcr) technique was demonstrat...

We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establishment of high-throughput assays. The different assays allow the analysis of strong and weak inte...

Understanding protein–ligand interactions in a cellular context is an important goal molecular biology and biochemistry, particularly for drug development. Investigators must demonstrate that drugs penetrate cells specifically bind their targets. Towards end, we present native mass spectrometry (MS)-based method analyzing uptake target engagement eukaryotic cells. This based on our previously i...

In order to maintain cellular homeostasis, cellular proteins coexist in complex and variable molecular assemblies. Therefore, understanding of major physiological processes at molecular level is based on analysis of protein-protein interaction networks. Firstly, composition of the molecular assembly has to be qualitatively analyzed. In the next step, quantitative bio-chemical properties of the ...