BACKGROUND During oncologic surgery, intraoperative manipulation of tumor tissue is almost unpreventable and causes a high risk of tumor cell dissemination into venous blood. A tumor cell-reducing effect of leukocyte adhesion filter systems has been shown under in vitro conditions. METHODS In a first clinical attempt, leukocyte adhesion filters were integrated into veno-venous bypass systems ...

BACKGROUND Vapocoolant sprays are commonly used to minimize pain following minor interventions such as venipuncture, shave biopsy or needle insertion. Although these sprays have been widely used in clinical practice, little is known about their effect on microcirculation or cutaneous blood flow. OBJECTIVE To evaluate the real-time effect of a topical vapocoolant using a well-established, rat ...

kcnk2 was previously shown to regulate immune-cell trafficking into the central nervous system (cns). kcnk2-/- mice demonstrated a more severe disease course in experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis, due to an increased immune-cell migration into the cns. an upregulation of the cellular adhesion molecules icam1 and vcam1 on brain endothelial cells in k...

background: recurrent spontaneous abortion (rsa) is defined as three or more se-quential abortions before the twentieth week of gestation. there are evidences to sup-port an allo-immunologic mechanism for rsa. one of the methods for treatment of rsa is leukocyte therapy; however there is still controversy about effectiveness of this method. objectives: to evaluate the effectiveness of leukocyte...

a simple special protocol using semichon’s acetic carmine stain was applied for examination of muscles of some small mammals, naturally infected with sarcocystis spp. using this method, infected muscles fibers with sarcocystis could easily be differentiated from muscle tissues. this method can be applied for screening of infected muscles with sarcocystis cysts on large number of samples for epi...

spontaneous abortion is the most common complication of pregnancy. several lines of evidence indicate that immunologic effector cells may play a role in the pathogenesis of idiopathic repetitive abortions. leukocytes form a substantial proportion of stromal cells in decidua and the endometrial granulated lymphocytes (egl) is the predominant decidual leukocyte population in the first trimester o...

OBJECTIVES Pyrophosphate arthropathy has been linked to diverse clinical subtypes. The two most common are: acute synovitis (pseudogout) and chronic pyrophosphate arthropathy ("pseudo-osteoarthritis"). We have conducted a study to examine whether these are overlapping syndromes. METHODS We reviewed all synovial fluid (SF) analyses performed in our laboratory from January 1988 to May 1997 to d...

OBJECTIVE To evaluate the role of rapamycin (RAPA) in paraquat (PQ)-induced acute lung injury. METHODS Lung tissues were stained with HE and lung histology was observed. Mortality rate, and neutrophil and leukocyte count in blood and bronchoalveolar lavage fluid (BALF) were recorded. Protein content in BALF was determined by Coomassie blue staining. Malondialdehyde (MDA) content, glutathione ...

Studies of innate immunity in fish species of special concern are essential for better understanding of their health status during hatchery rearing conditions. The cytochemical and morphological characterizations of blood granulocytes have been used to provide information about phylogenetic differences and determine the potential use of neutrophil function assays. Rapid, simple, cytochemical st...