We used combinatorial engineering to investigate the relationships between structure and linkage specificity of the dextransucrase DSR-S from Leuconostoc mesenteroides NRRL B-512F, and to generate variants with altered specificity. Sequence and structural analysis of glycoside-hydrolase family 70 enzymes led to eight amino acids (D306, F353, N404, W440, D460, H463, T464 and S512) being targeted...

A study of the influence of different plant terpenoids and amino sugar derivate acarbose on the activity of glycosyltransferase complex and purified dextransucrase from Leuconostoc mesenteroides URE 13 strain was carried out. All the tested terpenoids showed an inhibitory effect on glycosyltransferases from strain URE 13 at concentration 0.34 mmol. Out of all studied diterpenoids splendidin sho...

Leuconostoc species are Gram-positive, non-motile, vancomycin-resistant bacteria placed within the family of Streptococcaceae. They naturally exist in food and are important in the sauerkraut, milk and wine industries due to their role in fermentation. Infections caused by Leuconostocs are generally reported in immunosuppressed patients with an underlying disease, or in those who were previousl...

A study on the production of soluble and cell wall-bound glucansucrases (GS) and fructansucrases (FS) by Leuconostoc mesenteroides URE13 and L. mesenteroides Lm17 during their growth on media containing sucrose, glucose or fructose as a main carbon source was carried out. The changes in the enzyme activity, the assimilation of sugars and the formation of metabolites during the cultivation were ...

Aims: This study was conducted to isolate, identify and screen microorganisms associated with soy iru for the ability to be used as starter in the fermentation of soya bean (Glycine max) to produce soy iru. Study Design: To control the fermentation of soya bean (Glycine max) to produce soya iru using functional starter culture in order to improve the quality of the product. Place and Duration o...

The present study reports the complete genome sequence of Leuconostoc mesenteroides strain LT-38, which is a non-spore-forming Gram-positive lactic acid bacterium. The genome is composed of a 2,022,184-bp circular chromosome and contains 2,005 putative protein-coding genes.

In conjunction with investigations on the a-l, 6-glucosidic linkage in starch, it was desirable to conduct comparative studies on other substances containing this linkage. The dextran from Leuconostoc mesenteroides, a polysaccharide having predominantly a-1 ,6-glucosidic linkages, was selected to be used directly in these comparative studies and to provide a source of simpler substances contain...

Lactobacillus casei, a homofermentative lactic acid bacterium, was shown by Gibbs et al. (1950) to ferment glucose-i-C4 to products labeled as predicted for the classical EmbdenMeyerhof glycolytic scheme, whereas Leuconostoc mesenteroides strain 39, a heterofermentative lactic acid coccus, gave product labeling incompatible with an Embden-Meyerhof pathway and was interpreted as indicative of th...