An accumulated turbidity and the presence of many bacilli in the plasma surrounding lepromatous tissue culture explants has been emphasized by Timofejewsky (1) and by Suwo and Kin (2) as indicating the multiplication of free bacilli in the plasma itself. Since this inference seems unwarranted by the observations made during the preceding studies with tissue cultures, it is desired to explain wh...

The role of alpha-naphthaleneacetic acid (NAA) in the control of abscission in Citrus (Citrus sinensis L. Osbeck) leaf explants and its conjugation were studied in non-aged and 24-hour-aged explants. Dipping non-aged explants in 1.5 micromolar NAA for 15 minutes immediately after excision did not delay abscission whereas 150 micromolar NAA effectively delayed it. As incubation time was prolonge...

An efficient In vitro protocol was established for callogenesis from various plant parts (Leaf, stem, flower bud and tendril) of Passiflora vitifolia Kunth. On Murashige Skoog (MS) basal medium supplemented with concentration auxins (NAA, IAA, 2,4-D) cytokinins (BAP Kn ). Different concentrations (0.5 to 4 mg/L), IAA 2mg/L), NAA 2 mg/l) cytokinin BAP mg/L) were assessed callus induction. Growth...

* Author for correspondence: Rozi Mohamed, Department of Forest Management, Faculty of Forestry, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Tel.: 60-3-8946 7183 (Office), Fax: 60-3-8943 2514. Email: rozimohd@putra. upm.edu.my. Abstract. A critical stage in the introduction of plants into tissue culture is to obtain cultures free from microbial contamination. This study in...

Callus induction responses and regeneration through callus-mediated SE were studied from evening primrose (Oenothera biennis L.) as an important medicinal plant of Onagraceae mainly known for its gamma-linoleic acid (GLA) content. The effects of cytokinins, 6-Benzylaminopurine (BAP) and N-(2-furfurylamino)1-H-purine-6-amine [Kinetin (Kin)] and their concentrations (0, 0.5, 1.0 and 1.5 mg/l) in ...

Node, internode, and leaf explants of Populus deltoides Bartr. ex Marsh. × Populus deltoides Bartr. ex Marsh. hybrid poplar clone (89 M 066) tissue culture regenerated plantlets were used for direct and indirect somatic embryogenesis. Direct somatic embryos were differentiated on node, internode and leaf explants on Murashige and Skoog basal medium (MS) supplemented with N 6 -Benzyladenine (BA)...

In plants, multiple detached tissues are capable of forming a pluripotent cell mass, termed callus, when cultured on media containing appropriate plant hormones. Recent studies demonstrated that callus resembles the root-tip meristem, even if it is derived from aerial organs. This finding improves our understanding of the regeneration process of plant cells; however, the molecular mechanism tha...