Inflammation is an important reaction underlying lumbar disc herniation (LDH). Th17 cells play a critical role in immune activation. Interleukin (IL)-21 controls the functional activity of effector T-helper cells and the differentiation of Th17 cells, and promotes B-cell differentiation. It plays important roles in chronic inflammation and autoimmune diseases. However, little is known about rel...

PURPOSE This study evaluates the toxic effects of chrysene (a component from cigarette smoke) on Müller cells (MIO-M1) in vitro and investigates whether the inhibitor lipoic acid can reverse the chrysene-induced toxic effects. METHODS MIO-M1 cells were exposed to varying concentrations of chrysene with or without lipoic acid. Cell viability was measured by a trypan blue dye exclusion assay. C...

BACKGROUND Immunoassays for Plasmodium detection are, presently, most frequently based on monoclonal antibodies (MAbs); Polyclonal antibodies (PAbs), which are cheaper to develop and manufacture, are much less frequently used. In the present study we describe a sandwich ELISA assay which is capable of detecting P. vivax Lactate Dehydrogenase (LDH) in clinical blood samples, without cross reacti...

inflammation is an important reaction underlying lumbar disc herniation (ldh). th17 cells play a critical role in immune activation. interleukin (il)-21 controls the functional activity of effector t-helper cells and the differentiation of th17 cells, and promotes b-cell differentiation. it plays important roles in chronic inflammation and autoimmune diseases. however, little is known about rel...

Objective: Envenomation by heamotoxic snakes constituted a critical health occurrence in the world. Bleeding is the most sever consequence following snake bite with viperid and crothalid snakes. It is believed that the degradation of vascular membrane caused hemorrhage; in contrast, some suggested that direct cytotoxicity has role in endothelial cell disturbances. This study was to evaluate the...

The biological application of nanoparticles (NPs) is a rapidly developing area of nanotechnology that raises new possibilities in the treatment of human cancers. The cytotoxicity was evaluated by MTT and LDH assays. The apoptotic effect of free ICD-85 and ICD-85 NPs on HeLa cells was assessed using caspase-8 colorimetric assay. The MTT assay showed that ICD-85 NPs could enhance the in-vitro cyt...

PURPOSE The purpose of the present study was to investigate the role of glutathione peroxidase 4 (GPx4) in glutamate-induced oxytosis in the retina. METHODS For in vitro studies, an immortalized rat retinal precursor cell line R28 was used. Cells were transfected with siRNA specifically silencing GPx4 or with scrambled control siRNA. Lipid peroxidation was evaluated by 4-hydroxy-2-nonenal (4-...

Oxidative stress plays an important role in the pathogenesis of heart disease. Tocotrienol-rich fraction (TRF) is antioxidant and that has potential to reduce risk This study determine protective effects palm TRF against H2O2-induced oxidative damage neonatal rat cardiomyocytes (NRCM). The NRCM were divided into control, treated with (10 µg/mL), H2O2 (0.5 mM) prior induction (pre-treatment). Ce...

Objective: Envenomation by heamotoxic snakes constituted a critical health occurrence in the world. Bleeding is the most sever consequence following snake bite with viperid and crothalid snakes. It is believed that the degradation of vascular membrane caused hemorrhage; in contrast, some suggested that direct cytotoxicity has role in endothelial cell disturbances. This study was to evaluate the...

Introduction The aim of this study was to discuss the effects and mechanisms lncRNA in renal injury induced by diabetes vivo vitro study. Material methods Collecting healthy DN patients, measuring ANRIL expression RT-qPCR assay serum. Measuring normal, 10d 20d model rats kidney. LDH, MDA, SOD, IL-6 TNF-α concentration Elisa assay, evaluating kidney pathology HE staining, cell apoptosis TUNEL re...